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Journal of Clinical Microbiology, July 2003, p. 2849-2854, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2849-2854.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Randomly Amplified Polymorphic DNA as a Valuable Tool for Epidemiological Studies of Paracoccidioides brasiliensis

Rosane Christine Hahn,1 Andréa Mara Macedo,2 Cor Jesus Fernandes Fontes,3 Renata Dias Batista,1 Niriana Lara Santos,1 and Júnia Soares Hamdan1*

Departamento de Microbiologia, Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte,1 Departamento de Bioquímica e Imunologia Universidade Federal de Minas Gerais Belo Horizonte, Minas Gerais,2 Núcleo de Doenças Infecciosas e Tropicais, Trópica Faculdade de Ciências Médicas, Universidade Federal de Mato Grosso Cuiabá, Mato Grosso, Brazil3

Received 12 September 2002/ Returned for modification 24 November 2002/ Accepted 10 February 2003

Randomly amplified polymorphic DNA (RAPD) has been successfully used to detect genetic variations among isolates of Paracoccidioides brasiliensis. However, the usefulness of this technique for assessing important parasitic properties is still unconfirmed. In the present work we further investigated the applicability of RAPD in revealing important intrinsic and extrinsic features of this fungus associated with geographical origin, time of isolation, source of clinical specimen, clinical forms of human disease and also in vitro and in vivo susceptibility to antimicrobial and antifungal drugs. The RAPD patterns allowed us to distinguish all of the analyzed strains, which included 26 clinical isolates, 2 animal isolates, and 1 environmental isolate of P. brasiliensis obtained from different geographic regions, confirming the strong discriminating power of this technique. A phenetic tree, build from the RAPD data, showed that although the two nonclinical Brazilian strains were set together the majority of the clinical Brazilian strains were randomly distributed through different sub-branches of a major cluster without any correlation to any of the parameters analyzed. A second major cluster, however, has grouped isolates from Mato Grosso and Roraima (Brazil) that not only were susceptible in vitro to trimethoprim-sulfamethoxazole but also produced a good in vivo response. These results open new vistas for epidemiological and clinical studies of P. brasiliensis.

* Corresponding author. Mailing address: Departamento de Microbiologia, ICB/UFMG Av. Antônio Carlos, 6627 31270-901, Belo Horizonte Minas Gerais, Brazil. Phone: 31-3499-2758. Fax: 55-31-3499-2730. E-mail: handan{at}icb.ufmg.br.

Journal of Clinical Microbiology, July 2003, p. 2849-2854, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.2849-2854.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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