This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kikuchi, K.
Right arrow Articles by Uchiyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kikuchi, K.
Right arrow Articles by Uchiyama, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2003, p. 3001-3006, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3001-3006.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Strains Causing Neonatal Toxic Shock Syndrome-Like Exanthematous Disease in Neonatal and Perinatal Wards

Ken Kikuchi,1* Naoto Takahashi,2,{dagger} Chuncheng Piao,1 Kyoichi Totsuka,1 Hiroshi Nishida,2 and Takehiko Uchiyama1,3

Department of Infectious Diseases,1 Maternal and Perinatal Center,2 Department of Microbiology and Immunology, Tokyo Women's Medical University School of Medicine, Tokyo, Japan3

Received 16 December 2002/ Returned for modification 31 January 2003/ Accepted 31 March 2003

Neonatal toxic shock syndrome-like exanthematous disease (NTED) is a new neonatal disease caused by toxic shock syndrome toxin 1 (TSST-1). We conducted a prospective surveillance study and characterized the methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with NTED and compared them with the strains from patients with other MRSA infections and asymptomatic carriers. The study was performed in the neonatal intensive care unit and a general neonatal and maternal ward in the Tokyo Women's Medical University Hospital (TWMUH) from September to December 1998. Among 103 patients eligible for the study, MRSA was detected in 62 (60.2%) newborns; of these 62 newborns, 8 (12.9%) developed NTED, 1 (1.6%) had another MRSA infection, and 53 (85.5%) were asymptomatic MRSA carriers. Sixty-nine MRSA strains were obtained from the 62 newborns. DNA fingerprinting by pulsed-field gel electrophoresis showed two clusters: clone A with 8 subtypes and clone B. Sixty-seven of the 69 MRSA strains (97.1%) belonged to clone A, and type A1 was the most predominant (42 of 69 strains; 60.9%) in every neonatal and perinatal ward. All but one of the clone A strains had the TSST-1 and staphylococcal enterotoxin C genes. We also analyzed eight MRSA strains from eight NTED patients in five hospitals in Japan other than TWMUH. All the MRSA strains from NTED patients also belonged to clone A. These results suggest that a single clone that predominated in the neonatal wards of six hospitals might have caused NTED. However, the occurrence of NTED might not be dependent on the presence of an NTED-specific strain.

* Corresponding author. Mailing address: Department of Infectious Diseases, Tokyo Women's Medical University School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. Phone: 81-3-3353-8111, ext. 39321. Fax: 81-3-3358-8995. E-mail: kikuti{at}clabo.twmu.ac.jp.

{dagger} Present address: Department of Pediatrics, Jichi Medical School, Tochigi, Japan.

Journal of Clinical Microbiology, July 2003, p. 3001-3006, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3001-3006.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hoshi, S., Kikuchi, K., Sasaki, T., Sotozono, C., Kinoshita, S., Hiramatsu, K. (2008). Postantibiotic Effects and Bactericidal Activities of Levofloxacin and Gatifloxacin at Concentrations Simulating Those of Topical Ophthalmic Administration against Fluoroquinolone-Resistant and Fluoroquinolone-Sensitive Methicillin-Resistant Staphylococcus aureus Strains. Antimicrob. Agents Chemother. 52: 2970-2973 [Abstract] [Full Text]  
  • Parsonnet, J., Goering, R. V., Hansmann, M. A., Jones, M. B., Ohtagaki, K., Davis, C. C., Totsuka, K. (2008). Prevalence of Toxic Shock Syndrome Toxin 1 (TSST-1)-Producing Strains of Staphylococcus aureus and Antibody to TSST-1 among Healthy Japanese Women. J. Clin. Microbiol. 46: 2731-2738 [Abstract] [Full Text]  
  • Sasaki, T., Kikuchi, K., Tanaka, Y., Takahashi, N., Kamata, S., Hiramatsu, K. (2007). Reclassification of Phenotypically Identified Staphylococcus intermedius Strains. J. Clin. Microbiol. 45: 2770-2778 [Abstract] [Full Text]  
  • Sasaki, T., Kikuchi, K., Tanaka, Y., Takahashi, N., Kamata, S., Hiramatsu, K. (2007). Methicillin-Resistant Staphylococcus pseudintermedius in a Veterinary Teaching Hospital. J. Clin. Microbiol. 45: 1118-1125 [Abstract] [Full Text]  
  • Denis, O., Deplano, A., Nonhoff, C., Hallin, M., De Ryck, R., Vanhoof, R., De Mendonca, R., Struelens, M. J. (2006). In Vitro Activities of Ceftobiprole, Tigecycline, Daptomycin, and 19 Other Antimicrobials against Methicillin-Resistant Staphylococcus aureus Strains from a National Survey of Belgian Hospitals.. Antimicrob. Agents Chemother. 50: 2680-2685 [Abstract] [Full Text]  
  • Durand, G., Bes, M., Meugnier, H., Enright, M. C., Forey, F., Liassine, N., Wenger, A., Kikuchi, K., Lina, G., Vandenesch, F., Etienne, J. (2006). Detection of New Methicillin-Resistant Staphylococcus aureus Clones Containing the Toxic Shock Syndrome Toxin 1 Gene Responsible for Hospital- and Community-Acquired Infections in France.. J. Clin. Microbiol. 44: 847-853 [Abstract] [Full Text]  
  • Ramdani-Bouguessa, N., Bes, M., Meugnier, H., Forey, F., Reverdy, M.-E., Lina, G., Vandenesch, F., Tazir, M., Etienne, J. (2006). Detection of Methicillin-Resistant Staphylococcus aureus Strains Resistant to Multiple Antibiotics and Carrying the Panton-Valentine Leukocidin Genes in an Algiers Hospital. Antimicrob. Agents Chemother. 50: 1083-1085 [Abstract] [Full Text]  
  • Bratcher, D. (2005). Methicillin-resistant Staphylococcus aureus in Nurseries. NeoReviews 6: e424-e430 [Full Text]  
  • Takizawa, Y., Taneike, I., Nakagawa, S., Oishi, T., Nitahara, Y., Iwakura, N., Ozaki, K., Takano, M., Nakayama, T., Yamamoto, T. (2005). A Panton-Valentine Leucocidin (PVL)-Positive Community-Acquired Methicillin-Resistant Staphylococcus aureus (MRSA) Strain, Another Such Strain Carrying a Multiple-Drug Resistance Plasmid, and Other More-Typical PVL-Negative MRSA Strains Found in Japan. J. Clin. Microbiol. 43: 3356-3363 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Domelier, A.-S., Girard, N., Quentin, R., the Bloodstream Infection Study Group of the Relai, (2004). Epidemiology and Typing of Staphylococcus aureus Strains Isolated from Bloodstream Infections. J. Clin. Microbiol. 42: 5650-5657 [Abstract] [Full Text]  
  • Montague, E, Lynn, W, Michie, C A (2004). Umbilical disinfection: lessons from history. Arch. Dis. Child. Fetal Neonatal Ed. 89: F470-F470 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»