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Journal of Clinical Microbiology, July 2003, p. 3028-3034, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3028-3034.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Enteroviruses by Using Monoclonal Antibodies against a Putative Common Epitope

Soo-Youn Shin,1 Ki-Soon Kim,2 Yoon-Sung Lee,1 Yoon-Seok Chung,2 Kwi-Sung Park,1,2 Doo-Sung Cheon,2 Byoung-Kuk Na,2 Yoonsung Kang,1 Hyang-Min Cheong,1 Youngjoon Moon,1 Jee-Hye Choi,1 Hang-Eui Cho,1 Na-Young Min,1 Jin-Sook Son,1 Young-Hoon Park,1 Youngmee Jee,2 Jae-Deuk Yoon,2 Chul-Yong Song,1 and Kwang-Ho Lee1*

Department of Life Science, College of Natural Science, Chung-Ang University, Seoul 156-756,1 Laboratory of Enteroviruses, Department of Virology, National Institute of Health, Seoul 122-701, Korea2

Received 13 May 2002/ Returned for modification 7 January 2003/ Accepted 12 April 2003

A common epitope region of enteroviruses was identified by sequence-independent single-primer amplification (SISPA), followed by immunoscreening of 11 cDNA libraries from two Korean enterovirus isolates (echoviruses 7 and 30) and a coxsackievirus B3 (ATCC-VR 30). The putative common epitope region was localized in the N terminus of VP1 when the displayed recombinant proteins from the phages were chased by the convalescent-phase sera. The genomic region encoding the common epitope region was amplified and then expressed by using the vector pGEX-5X-1. The antigenicity of the expressed recombinant protein was identified by Western blotting with guinea pig antisera for six different serotypes of enteroviruses. After successive immunization of mice with the recombinant common epitope protein, splenocytes were extracted and hybridized with P3X63-Ag8-653 cells. A total of 24 hybridomas that produced monoclonal antibodies (MAbs) against the putative common epitope of enteroviruses were selected. Four of these were immunoglobulin G1 isotypes with a kappa light chain. These MAbs recognized 15 Korean endemic serotypes and prototypes of enteroviruses in an indirect immunofluorescence assay. These results suggest that the expressed protein might be a useful antigen for producing group common antibodies and that the use of the MAbs against the putative common epitope of enteroviruses might be a valuable diagnostic tool for rapidly identifying a broad range of enteroviruses.

* Corresponding author. Mailing address: Department of Life Science, College of Natural Science, Chung-Ang University, 221 Heuksukdong, Dongjakgu, Seoul 156-756, Korea. Phone: 82-2-820-5213. Fax: 82-2-824-9368. E-mail: leemanse{at}cau.ac.kr.

Journal of Clinical Microbiology, July 2003, p. 3028-3034, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3028-3034.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miao, L. Y., Pierce, C., Gray-Johnson, J., DeLotell, J., Shaw, C., Chapman, N., Yeh, E., Schnurr, D., Huang, Y. T. (2009). Monoclonal Antibodies to VP1 Recognize a Broad Range of Enteroviruses. J. Clin. Microbiol. 47: 3108-3113 [Abstract] [Full Text]  


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