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Journal of Clinical Microbiology, July 2003, p. 3056-3059, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3056-3059.2003

Field Evaluation of the gag-Based Heteroduplex Mobility Assay for Genetic Subtyping of Circulating Recombinant Forms of Human Immunodeficiency Virus Type 1 in Abidjan, Côte d'Ivoire

Souleymane Sawadogo,1 Christiane Adjé-Touré,1 Celestin E. Bilé,1 Rene E. A. Ekpini,1 Terence Chorba,1,2 and John N. Nkengasong1,3*

Projet RETRO-CI, Abidjan, Côte d'Ivoire,1 Division of HIV/AIDS Prevention, Surveillance and Epidemiology,3 Global AIDS Program, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 12 September 2002/ Returned for modification 29 November 2002/ Accepted 9 April 2003

The gag-based heteroduplex mobility assay (gag-HMA) was evaluated for its ease and reliability in subtyping circulating recombinant forms (CRFs) of human immunodeficiency virus type 1 (HIV-1) in Côte d'Ivoire. One hundred thirty-two plasma samples were analyzed blindly for HIV-1 subtypes by sequencing the pol gene and by gag-HMA. DNA sequencing was used as the "gold standard." Of the 132 samples sequenced, 108 (82%) were CRF02_AG, 14 (11%) were pure subtype A, 5 (4%) were subtype G, 3 (2%) were subtype D, 1 was CRF01_AE, and 1 was subtype H. The gag-HMA correctly classified 126 (95.5%) of the samples. Of the 108 samples that were classified as CRF02_AG by DNA sequencing, 107 (99%) were correctly identified by gag-HMA, resulting in a positive predictive value of 96.4%. The gag-HMA seems to be a valuable tool for understanding the molecular epidemiology of HIV-1 CRF02_AG in Côte d'Ivoire and West Africa, which could be important for developing and evaluating AIDS vaccines, although DNA sequencing remains necessary for accurate molecular epidemiology.

* Corresponding author. Mailing address: Division of HIV/AIDS Prevention, Surveillance and Epidemiology, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Ga. Phone: (404) 639-0871. Fax: (404) 639-2108. E-mail: jcn5{at}cdc.gov.

Journal of Clinical Microbiology, July 2003, p. 3056-3059, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3056-3059.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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