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Journal of Clinical Microbiology, July 2003, p. 3323-3326, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3323-3326.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing of Staphylococcus aureus with DNA Array Technology

Department of Medical Microbiology & Infectious Diseases, Erasmus MC, 3015 GD Rotterdam, The Netherlands,¹ bioMérieux, Marcy l'Etoile, France,² University of Bath, Bath, United Kingdom³

Received 15 January 2003/ Returned for modification 12 March 2003/ Accepted 11 April 2003

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

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