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Journal of Clinical Microbiology, July 2003, p. 3327-3330, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3327-3330.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Legionella pneumophila by Real-Time PCR for the mip Gene

Deborah A. Wilson,1 Belinda Yen-Lieberman,1 Udo Reischl,2 Steve M. Gordon,1 and Gary W. Procop1*

Cleveland Clinic Foundation, Cleveland, Ohio,1 Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany2

Received 6 December 2002/ Returned for modification 28 January 2003/ Accepted 3 April 2003

A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.

* Corresponding author. Mailing address: Department of Microbiology L40, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5944. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, July 2003, p. 3327-3330, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3327-3330.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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