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Journal of Clinical Microbiology, July 2003, p. 3327-3330, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3327-3330.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Legionella pneumophila by Real-Time PCR for the mip Gene

Cleveland Clinic Foundation, Cleveland, Ohio,¹ Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany²

Received 6 December 2002/ Returned for modification 28 January 2003/ Accepted 3 April 2003

A real-time PCR assay for the mip gene of Legionella pneumophila was tested with 27 isolates of L. pneumophila, 20 isolates of 14 other Legionella species, and 103 non-Legionella bacteria. Eight culture-positive and 40 culture-negative clinical specimens were tested. This assay was 100% sensitive and 100% specific for L. pneumophila.

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