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Journal of Clinical Microbiology, July 2003, p. 3331-3333, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3331-3333.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of a Vancomycin-Resistant Enterococcus faecalis (VREF) Isolate from a Dog with Mastitis: Further Evidence of a Clonal Lineage of VREF in New Zealand

Janet M. Manson, Stefanie Keis, John M. B. Smith, and Gregory M. Cook*

Department of Microbiology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand

Received 5 February 2003/ Returned for modification 23 March 2003/ Accepted 26 April 2003

We report here on the characterization of a vancomycin-resistant Enterococcus faecalis (VREF) isolated from a dog with mastitis. The isolate was positive for the vanA, ermB, and tet(M) genes, with vanA and ermB carried on the same transferable plasmid. Comparison of this isolate with VREF from poultry and human sources in New Zealand demonstrated identical SmaI macrorestriction patterns and Tn1546-like elements. This is further evidence of a clonal lineage of VREF in New Zealand.

* Corresponding author. Mailing address: Department of Microbiology, School of Medical Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64 3 479 7722. Fax: 64 3 479 8540. E-mail: greg.cook{at}stonebow.otago.ac.nz.

Journal of Clinical Microbiology, July 2003, p. 3331-3333, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3331-3333.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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