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Journal of Clinical Microbiology, July 2003, p. 3336-3338, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3336-3338.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Genotyping Helicobacter pylori Directly from Biopsy Specimens and Genotyping from Bacterial Cultures

Chang-Young Park,1,2 Minjung Kwak,3 Oscar Gutierrez,4 David Y. Graham,1 and Yoshio Yamaoka1*

Department of Medicine, Veterans Affairs Medical Center, and Baylor College of Medicine, Houston, Texas,1 Department of Internal Medicine, Kangbuk Samsung Hospital, Sunkyunkwan University School of Medicine, Seoul,2 Department of Information of Statistics, Pyongtaek University, Pyongtaek, Korea,3 Department of Medicine, Universidad Nacional de Colombia, Bogota, Colombia4

Received 10 December 2002/ Returned for modification 18 January 2003/ Accepted 21 April 2003

PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.

* Corresponding author. Mailing address: Veterans Affairs Medical Center (111D), 2002 Holcombe Blvd., Houston, TX 77030. Phone: (713) 794-7597. Fax: (713) 790-1040. E-mail: yyamaoka{at}bcm.tmc.edu.

Journal of Clinical Microbiology, July 2003, p. 3336-3338, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3336-3338.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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