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Journal of Clinical Microbiology, July 2003, p. 3361-3367, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3361-3367.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of the Clinical Sensitivities of Three Viral Load Assays with Plasma Samples from a Pediatric Population Predominantly Infected with Human Immunodeficiency Virus Type 1 Subtype G and BG Recombinant Forms

Rute Antunes,1,2 Sofia Figueiredo,1 Inês Bártolo,1,2 Manuel Pinheiro,3 Lino Rosado,4 Isabel Soares,5 Helena Lourenço,1 and Nuno Taveira1,2*

Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa,1 Unidade de Infecciologia Pediátrica, Hospital de Santa Maria de Lisboa,3 Unidade de Imunohematologia, Hospital Dona EstefÂnia, Lisbon,4 Instituto Superior de Ciências da Saúde Sul, Caparica,2 Unidade de Pediatria, Hospital Garcia de Orta, Almada, Portugal5

Received 22 October 2002/ Returned for modification 4 March 2003/ Accepted 9 April 2003

The viral load assays AMPLICOR HIV-1 Monitor Test 1.5, Nuclisens HIV-1 QT, and Quantiplex HIV RNA 3.0 (bDNA) were evaluated for their abilities to quantify human immunodeficiency virus type 1 (HIV-1) RNA in 64 plasma samples from 21 children infected in Portugal. The children were infected with HIV-1 subtypes A1, B, F1, G, and BG recombinant virus. AMPLICOR v1.5 and Quantiplex v3.0 detected all samples, and there was a good correlation of results between the two kits. Thirty-eight specimens containing HIV-1 subtype B, G, or recombinant BG, could not be detected by Nuclisens HIV-1 QT. We also evaluated the new Retina HIV-1 assay on 21 samples that were HIV-1 positive; Retina HIV-1 failed to detect 5 of 11 subtype G specimens. AMPLICOR v1.5 and Quantiplex v3.0 assays may be used for HIV-1 RNA quantification in Portugal, whereas an improvement in sensitivity for subtype G and recombinant BG is required for Nuclisens HIV-1 QT and Retina HIV-1.

* Corresponding author. Mailing address: Centro de Patogénese Molecular, Faculdade de Farmácia de Lisboa, Avenida das Forças Armadas, 1649-019 Lisbon, Portugal. Phone: 351 217 934 212. Fax: 351 217 934 212. E-mail: ntaveira{at}mail.telepac.pt.

Journal of Clinical Microbiology, July 2003, p. 3361-3367, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3361-3367.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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