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Journal of Clinical Microbiology, July 2003, p. 3407-3409, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3407-3409.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,¹ Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 06504²

Received 1 August 2002/ Returned for modification 29 November 2002/ Accepted 11 April 2003

To provide 24-h influenza diagnosis for adults presenting to the emergency department, the Directigen Flu A+B enzyme immunoassay (EIA) was performed in the chemistry laboratory during the night shift. Nasopharyngeal swabs were retested by cytospin-enhanced direct immunofluorescence (DFA; SimulFluor respiratory screen) when the virology laboratory opened. The influenza EIA detected 16 influenza A virus infections, whereas cytospin-enhanced DFA detected 31 influenza A virus infections as well as 3 respiratory syncytial virus, 2 adenovirus, and 1 parainfluenza virus infections. A positive EIA result usually correlated with 50 or more influenza virus cells positive by DFA.

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