This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Segonds, C. Articles by Chabanon, G. Search for Related Content PubMed PubMed Citation Articles by Segonds, C. Articles by Chabanon, G. Pubmed/NCBI databases Medline Plus Health Information Cystic Fibrosis

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2003, p. 3415-3418, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3415-3418.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Amplified Ribosomal DNA Restriction Analysis for Identification of Ralstonia and Pandoraea Species: Interest in Determination of the Respiratory Bacterial Flora in Patients with Cystic Fibrosis

Christine Segonds,^* Sandrine Paute, and Gérard Chabanon

Observatoire Cepacia/Vaincre la Mucoviscidose, Laboratoire de Bactériologie-Hygiène, Hôpital Rangueil-TSA 50032, 31059 Toulouse Cedex 9, France

Received 7 November 2002/ Returned for modification 21 January 2003/ Accepted 24 April 2003

The recovery of Ralstonia and Pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. These species are difficult to identify, and especially to differentiate from Burkholderia cepacia complex organisms, with classical methods. The discriminatory power of amplified ribosomal DNA restriction analysis (ARDRA) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six enzymes already proven suitable for the identification of Burkholderia species. ARDRA provided differentiation of all the Ralstonia species tested and of Pandoraea norimbergensis. Pandoraea species P. pnomenusa, P. sputorum, P. pulmonicola, and P. apista were not discriminated to the species level. This method allowed the identification of five clinical isolates recovered from French cystic fibrosis patients as Ralstonia mannitolilytica.

---

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Hygiène, Hôpital Rangueil-TSA 50032, 31059 Toulouse Cedex 9, France. Phone: 33-(0)5-61-32-28-28. Fax: 33-(0)5-61-32-26-20. E-mail: segonds{at}cict.fr.

---

Journal of Clinical Microbiology, July 2003, p. 3415-3418, Vol. 41, No. 7
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.7.3415-3418.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Degand, N., Carbonnelle, E., Dauphin, B., Beretti, J.-L., Le Bourgeois, M., Sermet-Gaudelus, I., Segonds, C., Berche, P., Nassif, X., Ferroni, A. (2008). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis Patients. J. Clin. Microbiol. 46: 3361-3367 [Abstract] [Full Text]  
• Pimentel, J. D., MacLeod, C. (2008). Misidentification of Pandoraea sputorum Isolated from Sputum of a Patient with Cystic Fibrosis and Review of Pandoraea Species Infections in Transplant Patients. J. Clin. Microbiol. 46: 3165-3168 [Abstract] [Full Text]  
• Aravena-Roman, M. (2008). Cellular fatty acid-deficient Pandoraea isolated from a patient with cystic fibrosis. J Med Microbiol 57: 252-252 [Full Text]  
• Atkinson, R. M., LiPuma, J. J., Rosenbluth, D. B., Dunne, W. M. Jr. (2006). Chronic Colonization with Pandoraea apista in Cystic Fibrosis Patients Determined by Repetitive-Element-Sequence PCR.. J. Clin. Microbiol. 44: 833-836 [Abstract] [Full Text]  
• Chiron, R., Marchandin, H., Counil, F., Jumas-Bilak, E., Freydiere, A.-M., Bellon, G., Husson, M.-O., Turck, D., Bremont, F., Chabanon, G., Segonds, C. (2005). Clinical and Microbiological Features of Inquilinus sp. Isolates from Five Patients with Cystic Fibrosis. J. Clin. Microbiol. 43: 3938-3943 [Abstract] [Full Text]