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Journal of Clinical Microbiology, August 2003, p. 3566-3573, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3566-3573.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evidence for Genetic Linkage between the Gene Segments Encoding NSP4 and VP6 Proteins in Common and Reassortant Human Rotavirus Strains

Miren Iturriza-Gòmara,1* Emma Anderton,2 Gagandeep Kang,3 Chris Gallimore,1 Wendy Phillips,2 Ulrich Desselberger,2,{dagger} and Jim Gray1

Enteric Virus Unit, Enteric, Respiratory and Neurological Virus Laboratory, Health Protection Agency, London,1 Clinical Microbiology Laboratory, Addenbrooke's Hospital, Cambridge, United Kingdom,2 Department of Gastrointestinal Sciences, Christian Medical College and Hospital, Vellore 632004, India3

Received 27 February 2003/ Returned for modification 14 May 2003/ Accepted 22 May 2003

NSP4-encoding genes of 78 human rotavirus strains of common or reassortant genotypes were characterized by reverse transcription-PCR followed by sequencing and phylogenetic analysis. It was found that all the human strains characterized clustered into only two of the five known NSP4 genotypes. Linkage between NSP4 genotypes and VP6 subgroups was 100%, NSP4 genotype A being linked to VP6 of subgroup I (SGI) and NSP4 of genotype B being linked to VP6 of SGII. The diversity among the NSP4- and VP6-encoding genes was significantly less than that among the VP7 and VP4 genes in cocirculating human rotavirus strains. Whereas G and P types appear to be shared among different animal species and humans, the NSP4- and VP6-encoding genes appear to segregate according to their host of origin, suggesting that these two proteins may be host restriction determinants. The NSP4-VP6 association may be structurally determined during rotavirus replication (morphogenesis).

* Corresponding author. Mailing address: Enteric Virus Unit, Enteric Respiratory and Neurological Virus Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 (0)20 82004400. Fax: 44 (0)20 82058195. E-mail: MIturriza{at}phls.org.uk.

{dagger} Present address: Virologie Molèculaire et Structurale UMR 2472, CNRS, Gif-sur-Yvette Cedex, France.

Journal of Clinical Microbiology, August 2003, p. 3566-3573, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3566-3573.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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