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Journal of Clinical Microbiology, August 2003, p. 3579-3583, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3579-3583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Avian Metapneumovirus Type C-Specific Antibodies in Multiple Domestic Avian Species

Elizabeth A. Turpin,1,2 Dale C. Lauer,3 and David E. Swayne1*

Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605,1 Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602,2 Minnesota Poultry Testing Laboratory, Willmar, Minnesota 562013

Received 2 October 2002/ Returned for modification 26 March 2003/ Accepted 20 May 2003

The first cases of infection caused by avian metapneumoviruses (aMPVs) were described in turkeys with respiratory disease in South Africa during 1978. The causative agent was isolated and identified as a pneumovirus in 1986. aMPVs have been detected in domestic nonpoultry species in Europe, but tests for the detection of these viruses are not available in the United States. To begin to understand the potential role of domestic ducks and geese and wild waterfowl in the epidemiology of aMPV, we have developed and evaluated a blocking enzyme-linked immunosorbent assay (bELISA) for the detection of aMPV type C (aMPV-C)-specific antibodies. This assay method overcomes the species-specific platform of indirect ELISAs to allow detection of aMPV-C-specific antibodies from potentially any avian species. The bELISA was initially tested with experimental turkey serum samples, and the results were found to correlate with those of virus neutralization assays and indirect enzyme-linked immunosorbent assay (iELISA). One thousand serum samples from turkey flocks in Minnesota were evaluated by our bELISA, and the level of agreement of the results of the bELISA and those of the iELISA was 94.9%. In addition, we were able to show that the bELISA could detect aMPV-C-specific antibodies from experimentally infected ducks, indicating its usefulness for the screening of serum samples from multiple avian species. This is the first diagnostic assay for the detection of aMPV-C-specific antibodies from multiple avian species in the United States.

* Corresponding author. Mailing address: Southeast Poultry Research Laboratory, ARS, USDA, Athens, GA 30605. Phone: (706) 546-3433. Fax: (706) 546-3161. E-mail: dswayne{at}seprl.usda.gov.

Journal of Clinical Microbiology, August 2003, p. 3579-3583, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3579-3583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alvarez, R., Njenga, M. K., Scott, M., Seal, B. S. (2004). Development of a Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay Using a Synthetic Peptide Antigen for Detection of Avian Metapneumovirus Antibodies in Turkey Sera. CVI 11: 245-249 [Abstract] [Full Text]  


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