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Journal of Clinical Microbiology, August 2003, p. 3609-3614, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3609-3614.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Coagulase-Negative Staphylococci: Comparison of Phenotypic and Genotypic Oxacillin Susceptibility Tests and Evaluation of the Agar Screening Test by Using Different Concentrations of Oxacillin

Rosana B. R. Ferreira,1 Natalia L. P. Iorio,1 Karoline L. Malvar,1 Ana Paula F. Nunes,1 Leila S. Fonseca,1 Carla C. R. Bastos,2 and Kátia R. N. Santos1*

Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro,1 Marcílio Dias Naval Hospital, Rio de Janeiro, Brazil2

Received 8 January 2003/ Returned for modification 24 April 2003/ Accepted 2 June 2003

This study evaluated the oxacillin susceptibilities of 152 coagulase-negative staphylococcal (CoNS) strains of 12 species by disk diffusion; agar dilution; E-test; the slide latex agglutination test (Slidex MRSA Detection test; bioMérieux S/A, Paris, France); the agar screening test with 1, 2, 4, or 6 µg of oxacillin per ml and incubation for 24 or 48 h; and detection of the mecA gene by PCR. The results revealed that the agar screening test with 4 µg of oxacillin per ml and incubation for 48 h was superior to any single phenotype-based susceptibility assay, presenting a sensitivity and a specificity of 100% each. For the different methods evaluated, the sensitivities and specificities were as follows: for disk diffusion, 94.2 and 91.8%, respectively; for the agar dilution test 100 and 73.5%, respectively; for E-test, 100 and 71.4%, respectively; and for the slide latex agglutination test, 97.1 and 98%, respectively. A good correlation was observed between oxacillin susceptibility testing results and PCR results for Staphylococcus epidermidis, S. haemolyticus, S. hominis subsp. hominis, and all mecA-positive strains. However, at least 60% of the mecA-negative isolates of the species S. saprophyticus, S. cohnii subsp. urealyticum, S. lugdunensis, and S. sciuri were erroneously classified as oxacillin resistant by the agar dilution test. Conversely, the slide latex agglutination test presented a high sensitivity (97.1%) and a high specificity (98%) for all CoNS species. Our results demonstrated the accuracy of the agar screening test with 4 µg of oxacillin per ml and incubation for 48 h and the slide latex agglutination test for the appropriate detection of the oxacillin susceptibilities of CoNS isolates. Both assays are technically simple and can be easier to perform in routine laboratories than PCR.

* Corresponding author. Mailing address: Laboratório de Infecções Hospitalares, Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Bloco I, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil, CEP: 21941-590. Phone and fax: 55-21-2560-8028. E-mail: santoskrn{at}micro.ufrj.br.

Journal of Clinical Microbiology, August 2003, p. 3609-3614, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3609-3614.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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