This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boyd, E. F.
Right arrow Articles by McClelland, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boyd, E. F.
Right arrow Articles by McClelland, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2003, p. 3823-3828, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3823-3828.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differences in Gene Content among Salmonella enterica Serovar Typhi Isolates

E. F. Boyd,{dagger} S. Porwollik,* F. Blackmer, and M. McClelland

Sidney Kimmel Cancer Center, San Diego, California 92121

Received 24 March 2003/ Accepted 13 May 2003

We used a nonredundant microarray of the Salmonella enterica serovar Typhimurium LT2 and Typhi CT18 genomes to assess the genomic content of a diverse set of isolates of serovar Typhi. Comparative genomic hybridization revealed 13 regions of absent or divergent gene content in the eight Typhi strains examined compared to Typhi CT18. In particular, two Typhi CT18 prophage regions, STY1048 to STY1077 and STY2038 to STY2077, as well as a five-gene islet (STY3188 to STY3193) were absent or divergent in all other Typhi strains examined. Seven Typhi strains lacked most or all of the IS1 elements present in strain CT18, and three Typhi strains lacked a P4-like phage (STY4821 to STY4834). One strain was devoid of a 149-gene region (STY4521 to STY4680), which encodes numerous phage genes and the Vi antigen biosynthesis and export gene cluster, a type IV pilus, and numerous phage genes. In Typhi strain 26T25, an amplification of an entire inter-ribosomal region encompassing 31 genes has occurred. Furthermore, a 257-gene region (STY1360 to STY1639) showed an aberrant replication pattern in three Typhi isolates. Overall, these differences in gene content indicate that even within a highly clonal bacterial population the genomic reservoir is unstable.

* Corresponding author. Mailing address: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121. Phone: (858) 450-5990. Fax: (858) 550-3998. E-mail: sporwollik{at}skcc.org.

{dagger} Present address: Department of Microbiology, UCC, National University of Ireland, Cork, Ireland.

Journal of Clinical Microbiology, August 2003, p. 3823-3828, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3823-3828.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Leader, B. T., Frye, J. G., Hu, J., Fedorka-Cray, P. J., Boyle, D. S. (2009). High-Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis. J. Clin. Microbiol. 47: 1290-1299 [Abstract] [Full Text]  
  • Arrach, N., Porwollik, S., Cheng, P., Cho, A., Long, F., Choi, S.-H., McClelland, M. (2008). Salmonella Serovar Identification Using PCR-Based Detection of Gene Presence and Absence. J. Clin. Microbiol. 46: 2581-2589 [Abstract] [Full Text]  
  • Heithoff, D. M., Shimp, W. R., Lau, P. W., Badie, G., Enioutina, E. Y., Daynes, R. A., Byrne, B. A., House, J. K., Mahan, M. J. (2008). Human Salmonella Clinical Isolates Distinct from Those of Animal Origin. Appl. Environ. Microbiol. 74: 1757-1766 [Abstract] [Full Text]  
  • Badie, G., Heithoff, D. M., Sinsheimer, R. L., Mahan, M. J. (2007). Altered Levels of Salmonella DNA Adenine Methylase Are Associated with Defects in Gene Expression, Motility, Flagellar Synthesis, and Bile Resistance in the Pathogenic Strain 14028 but Not in the Laboratory Strain LT2. J. Bacteriol. 189: 1556-1564 [Abstract] [Full Text]  
  • Faucher, Séb. P., Porwollik, S., Dozois, C. M., McClelland, M., Daigle, F. (2006). Transcriptome of Salmonella enterica serovar Typhi within macrophages revealed through the selective capture of transcribed sequences. Proc. Natl. Acad. Sci. USA 103: 1906-1911 [Abstract] [Full Text]  
  • McMeechan, A., Lovell, M. A., Cogan, T. A., Marston, K. L., Humphrey, T. J., Barrow, P. A. (2005). Glycogen production by different Salmonella enterica serotypes: contribution of functional glgC to virulence, intestinal colonization and environmental survival. Microbiology 151: 3969-3977 [Abstract] [Full Text]  
  • Bishop, A. L., Baker, S., Jenks, S., Fookes, M., Gaora, P. O, Pickard, D., Anjum, M., Farrar, J., Hien, T. T., Ivens, A., Dougan, G. (2005). Analysis of the Hypervariable Region of the Salmonella enterica Genome Associated with tRNAleuX. J. Bacteriol. 187: 2469-2482 [Abstract] [Full Text]  
  • Reen, F. J., Boyd, E. F., Porwollik, S., Murphy, B. P., Gilroy, D., Fanning, S., McClelland, M. (2005). Genomic Comparisons of Salmonella enterica Serovar Dublin, Agona, and Typhimurium Strains Recently Isolated from Milk Filters and Bovine Samples from Ireland, Using a Salmonella Microarray. Appl. Environ. Microbiol. 71: 1616-1625 [Abstract] [Full Text]  
  • Porwollik, S., Boyd, E. F., Choy, C., Cheng, P., Florea, L., Proctor, E., McClelland, M. (2004). Characterization of Salmonella enterica Subspecies I Genovars by Use of Microarrays. J. Bacteriol. 186: 5883-5898 [Abstract] [Full Text]  
  • Le, T. A. H., Lejay-Collin, M., Grimont, P. A. D., Hoang, T. L., Nguyen, T. V., Grimont, F., Scavizzi, M. R. (2004). Endemic, Epidemic Clone of Salmonella enterica Serovar Typhi Harboring a Single Multidrug-Resistant Plasmid in Vietnam between 1995 and 2002. J. Clin. Microbiol. 42: 3094-3099 [Abstract] [Full Text]  
  • Bueno, S. M., Santiviago, C. A., Murillo, A. A., Fuentes, J. A., Trombert, A. N., Rodas, P. I., Youderian, P., Mora, G. C. (2004). Precise Excision of the Large Pathogenicity Island, SPI7, in Salmonella enterica Serovar Typhi. J. Bacteriol. 186: 3202-3213 [Abstract] [Full Text]  
  • Nair, S., Alokam, S., Kothapalli, S., Porwollik, S., Proctor, E., Choy, C., McClelland, M., Liu, S.-L., Sanderson, K. E. (2004). Salmonella enterica Serovar Typhi Strains from Which SPI7, a 134-Kilobase Island with Genes for Vi Exopolysaccharide and Other Functions, Has Been Deleted. J. Bacteriol. 186: 3214-3223 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»