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Journal of Clinical Microbiology, August 2003, p. 3846-3850, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3846-3850.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of a Modified Single-Enzyme Amplified-Fragment Length Polymorphism Technique for Fingerprinting and Differentiating of Mycobacterium kansasii Type I Isolates

Ayman Gaafar,1 M. Josebe Unzaga,1* Ramón Cisterna,1 Felicitas Elena Clavo,2 Elena Urra,3 Rafael Ayarza,4 and Gloria Martín5

Servicio Microbiología, Hospital de Basurto,1 Hospital de Santa Marina,2 Hospital de Cruces,3 Hospital de Galdakao,4 Hospital de San Eloy, Bilbao, Spain5

Received 18 March 2003/ Returned for modification 16 April 2003/ Accepted 27 May 2003

The usefulness of single-enzyme amplified-fragment length polymorphism (AFLP) analysis for the subtyping of Mycobacterium kansasii type I isolates was evaluated. This simplified technique classified 253 type I strains into 12 distinct clusters. The discriminating power of this technique was high, and the technique easily distinguished between the epidemiologically unrelated control strains and our clinical isolates. Overall, the technique was relatively rapid and technically simple, yet it gave reproducible and discriminatory results. This technique provides a powerful typing tool which may be helpful in solving many questions concerning the reservoirs, pathogenicities, and modes of transmission of these isolates.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital de Basurto, Avda. Montevideo 18, 48013 Bilbao, Spain. Phone: (34) 944006017. Fax: (34) 944006185. E-mail: munzaga{at}hbas.osakidetza.net.

Journal of Clinical Microbiology, August 2003, p. 3846-3850, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3846-3850.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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