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Journal of Clinical Microbiology, August 2003, p. 3857-3860, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3857-3860.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Diversity among Escherichia coli O157:H7 Isolates from Bovines Living on Farms in England and Wales

Ernesto Liebana,1* Richard P. Smith,2 Elisabeth Lindsay,3 Ian McLaren,1 Claire Cassar,1 Felicity A. Clifton-Hadley,1 and Giles A. Paiba2

Department of Bacterial Diseases,1 Epidemiology Department, Veterinary Laboratories Agency-Weybridge, Addlestone, Surrey KT15 3NB,2 Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT, United Kingdom3

Received 2 October 2002/ Returned for modification 12 January 2003/ Accepted 8 May 2003

Pulsed-field gel electrophoresis of Escherichia coli O157:H7 isolates (n = 228) from 122 healthy animals on 11 farms discriminated 57 types. Most clones were found only on individual farms. Numerous clones were found within each farm, with a prevalent clone normally found in several animals. A variety of clones were found within the different phage types.


* Corresponding author. Mailing address: Veterinary Laboratories Agency-Weybridge, Department of Bacterial Diseases, Woodham Lane, Addlestone, KT15 3NB Surrey, England, United Kingdom. Phone: 44 1932 357587. Fax: 44 1932 357595. E-mail: E.liebana{at}VLA.DEFRA.gsi.gov.UK.


Journal of Clinical Microbiology, August 2003, p. 3857-3860, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3857-3860.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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