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Journal of Clinical Microbiology, August 2003, p. 3861-3863, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3861-3863.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Candida glabrata with a New Commercial Test, GLABRATA RTT

Laboratoire de Bacteriologie, Hôpital Debrousse, Lyon,¹ Groupe d'Étude des Interactions Hôte-Parasite, Angers,² SR2B, Avrillé, France³

Received 7 January 2003/ Returned for modification 20 March 2003/ Accepted 13 May 2003

The GLABRATA RTT test (Fumouze Diagnostics, Levallois Perret, France) is based on the ability of Candida glabrata to hydrolyze trehalose but not maltose. It requires an inoculum of only four to six colonies, and the results are available within 20 min. We tested GLABRATA RTT with 330 stock isolates grown in subcultures on four different primary fungal isolation media and obtained a sensitivity of 94 to 98% (depending on the medium used) and a specificity of 97.3 to 98.6%. The false-positive results corresponded to C. tropicalis, C. famata, and C. lusitaniae. GLABRATA RTT thus offers rapid and reliable identification of C. glabrata.

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