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Quantitative Real-Time PCR Assay for Detection of Ehrlichia chaffeensis

Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 25 February 2003/ Returned for modification 24 April 2003/ Accepted 7 May 2003

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10¹⁰ gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

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