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Journal of Clinical Microbiology, August 2003, p. 3893-3896, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3893-3896.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

VIM-1 Metallo-ß-Lactamase-Producing Klebsiella pneumoniae Strains in Greek Hospitals

Departments of Hygiene and Epidemiology,¹ Microbiology, Medical School, University of Athens,⁶ Hippokration General Hospital,² Sismanoglio General Hospital,³ G. Genimatas General Hospital,⁴ Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece⁵

Received 3 March 2003/ Returned for modification 18 April 2003/ Accepted 15 May 2003

Seventeen Klebsiella pneumoniae clinical isolates carrying the bla_VIM-1 metallo-ß-lactamase gene were collected in the intensive care units of three hospitals in Athens, Greece, in 2002. They exhibited various carbapenem resistance levels (Etest MICs of imipenem ranged from 4 to 32 µg/ml). All isolates gave positive results by the imipenem-EDTA synergy Etest. The isolates were classified into four main types by pulsed-field gel electrophoresis; the majority of the isolates (5 and 10 isolates) belonged to two types. The bla_VIM-1 gene cassette was part of the variable region of a class 1 integron that also included aac6, dhfrI, and aadA. This structure was carried by transferable plasmids.

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