This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sanguinetti, M.
Right arrow Articles by Fadda, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sanguinetti, M.
Right arrow Articles by Fadda, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2003, p. 3922-3925, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3922-3925.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Real-Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme-Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis

Maurizio Sanguinetti,1* Brunella Posteraro,1 Livio Pagano,2 Gabriella Pagliari,3 Luana Fianchi,2 Luca Mele,2 Marilena La Sorda,1 Angelica Franco,1 and Giovanni Fadda1

Istituto di Microbiologia,1 Istituto di Semeiotica Medica-Cattedra di Ematologia,2 Istituto di Fisiopatologia Respiratoria, Università Cattolica del Sacro Cuore, Rome, Italy3

Received 25 February 2003/ Returned for modification 24 April 2003/ Accepted 26 May 2003

An iCycler iQ real-time PCR assay targeting 18S rRNA Aspergillus-specific sequences was developed for the diagnosis of invasive pulmonary aspergillosis (IPA). Positive findings were obtained for 18 of 20 (90%) bronchoalveolar lavage (BAL) fluid specimens from patients with probable or confirmed IPA and were obtained for none of the 24 BAL samples from patients with no clinical evidence of aspergillosis. These results were concordant with those of a nested PCR assay, which detected 90% of the patients with IPA, while galactomannan ELISA revealed positivity for 100% of these patients, suggesting that combined use of methods might improve the diagnosis of IPA.

* Corresponding author. Mailing address: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, L. go F. Vito, 1-00168 Rome, Italy. Phone: 39-0630154965. Fax: 39-063051152. E-mail: msanguinetti{at}rm.unicatt.it.

Journal of Clinical Microbiology, August 2003, p. 3922-3925, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3922-3925.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pagano, L., Fianchi, L., Caira, M. (2008). Pulmonary aspergillosis in hematologic malignancies: lights and shadows. haematol 93: 1611-1616 [Full Text]  
  • Penack, O., Rempf, P., Graf, B., Blau, I. W., Thiel, E. (2008). Aspergillus galactomannan testing in patients with long-term neutropenia: implications for clinical management. Ann Oncol 19: 984-989 [Abstract] [Full Text]  
  • Bulpa, P., Dive, A., Sibille, Y. (2007). Invasive pulmonary aspergillosis in patients with chronic obstructive pulmonary disease. Eur Respir J 30: 782-800 [Abstract] [Full Text]  
  • Nguyen, M. H., Jaber, R., Leather, H. L., Wingard, J. R., Staley, B., Wheat, L. J., Cline, C. L., Baz, M., Rand, K. H., Clancy, C. J. (2007). Use of Bronchoalveolar Lavage To Detect Galactomannan for Diagnosis of Pulmonary Aspergillosis among Nonimmunocompromised Hosts. J. Clin. Microbiol. 45: 2787-2792 [Abstract] [Full Text]  
  • Clancy, C. J., Jaber, R. A., Leather, H. L., Wingard, J. R., Staley, B., Wheat, L. J., Cline, C. L., Rand, K. H., Schain, D., Baz, M., Nguyen, M. H. (2007). Bronchoalveolar Lavage Galactomannan in Diagnosis of Invasive Pulmonary Aspergillosis among Solid-Organ Transplant Recipients. J. Clin. Microbiol. 45: 1759-1765 [Abstract] [Full Text]  
  • Schabereiter-Gurtner, C., Selitsch, B., Rotter, M. L., Hirschl, A. M., Willinger, B. (2007). Development of Novel Real-Time PCR Assays for Detection and Differentiation of Eleven Medically Important Aspergillus and Candida Species in Clinical Specimens. J. Clin. Microbiol. 45: 906-914 [Abstract] [Full Text]  
  • Francesconi, A., Kasai, M., Petraitiene, R., Petraitis, V., Kelaher, A. M., Schaufele, R., Hope, W. W., Shea, Y. R., Bacher, J., Walsh, T. J. (2006). Characterization and Comparison of Galactomannan Enzyme Immunoassay and Quantitative Real-Time PCR Assay for Detection of Aspergillus fumigatus in Bronchoalveolar Lavage Fluid from Experimental Invasive Pulmonary Aspergillosis.. J. Clin. Microbiol. 44: 2475-2480 [Abstract] [Full Text]  
  • Mennink-Kersten, M. A. S. H., Ruegebrink, D., Wasei, N., Melchers, W. J. G., Verweij, P. E. (2006). In Vitro Release by Aspergillus fumigatus of Galactofuranose Antigens, 1,3-{beta}-D-Glucan, and DNA, Surrogate Markers Used for Diagnosis of Invasive Aspergillosis.. J. Clin. Microbiol. 44: 1711-1718 [Abstract] [Full Text]  
  • Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R. III, Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clin. Microbiol. Rev. 19: 165-256 [Abstract] [Full Text]  
  • Halliday, C., Wu, Q. X., James, G., Sorrell, T. (2005). Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens. J. Clin. Microbiol. 43: 5366-5368 [Abstract] [Full Text]  
  • White, P. L., Archer, A. E., Barnes, R. A. (2005). Comparison of Non-Culture-Based Methods for Detection of Systemic Fungal Infections, with an Emphasis on Invasive Candida Infections. J. Clin. Microbiol. 43: 2181-2187 [Abstract] [Full Text]  
  • Musher, B., Fredricks, D., Leisenring, W., Balajee, S. A., Smith, C., Marr, K. A. (2004). Aspergillus Galactomannan Enzyme Immunoassay and Quantitative PCR for Diagnosis of Invasive Aspergillosis with Bronchoalveolar Lavage Fluid. J. Clin. Microbiol. 42: 5517-5522 [Abstract] [Full Text]  
  • Azzola, A., Passweg, J. R., Habicht, J. M., Bubendorf, L., Tamm, M., Gratwohl, A., Eich, G. (2004). Use of Lung Resection and Voriconazole for Successful Treatment of Invasive Pulmonary Aspergillus ustus Infection. J. Clin. Microbiol. 42: 4805-4808 [Abstract] [Full Text]  
  • Kawazu, M., Kanda, Y., Nannya, Y., Aoki, K., Kurokawa, M., Chiba, S., Motokura, T., Hirai, H., Ogawa, S. (2004). Prospective Comparison of the Diagnostic Potential of Real-Time PCR, Double-Sandwich Enzyme-Linked Immunosorbent Assay for Galactomannan, and a (1->3)-{beta}-D-Glucan Test in Weekly Screening for Invasive Aspergillosis in Patients with Hematological Disorders. J. Clin. Microbiol. 42: 2733-2741 [Abstract] [Full Text]  
  • Wu, Y. P., Wei, R., Verhoef, J. (2003). Real time assay of Aspergillus should be used in SARS patients receiving corticosteroids. BMJ 327: 1405-1405 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»