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Journal of Clinical Microbiology, August 2003, p. 3922-3925, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3922-3925.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Real-Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme-Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis

Istituto di Microbiologia,¹ Istituto di Semeiotica Medica-Cattedra di Ematologia,² Istituto di Fisiopatologia Respiratoria, Università Cattolica del Sacro Cuore, Rome, Italy³

Received 25 February 2003/ Returned for modification 24 April 2003/ Accepted 26 May 2003

An iCycler iQ real-time PCR assay targeting 18S rRNA Aspergillus-specific sequences was developed for the diagnosis of invasive pulmonary aspergillosis (IPA). Positive findings were obtained for 18 of 20 (90%) bronchoalveolar lavage (BAL) fluid specimens from patients with probable or confirmed IPA and were obtained for none of the 24 BAL samples from patients with no clinical evidence of aspergillosis. These results were concordant with those of a nested PCR assay, which detected 90% of the patients with IPA, while galactomannan ELISA revealed positivity for 100% of these patients, suggesting that combined use of methods might improve the diagnosis of IPA.

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