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Journal of Clinical Microbiology, August 2003, p. 3933-3935, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3933-3935.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Modification of the Double-Disk Test for Detection of Enterobacteriaceae Producing Extended-Spectrum and AmpC ß-Lactamases

J. D. D. Pitout,1,2* M. D. Reisbig,3 E. C. Venter,2 D. L. Church,1 and N. D. Hanson3

Division of Microbiology, Calgary Laboratory Services, and Department of Pathology & Laboratory Medicine, University of Calgary, Calgary T3C 0J5, Canada,1 Department of Medical Microbiology, University of the Free State, Bloemfontein, South Africa 9300,2 Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 681783

Received 26 September 2002/ Returned for modification 19 March 2003/ Accepted 4 May 2003

Detection of extended-spectrum ß-lactamases (ESBLs) in AmpC-producing Enterobacteriaceae is problematic. A modification of the double-disk test (MDDT) has been developed for successful detection of ESBLs in gram-negative bacilli producing well-characterized ß-lactamases as well as 212 clinical isolates of Enterobacter cloacae, Enterobacter aerogenes, Serratia marcescens, and Citrobacter freundii. MDDT accurately differentiated between ESBL producers and derepressed chromosomal AmpC mutants. MDDT provides a cost-effective alternative approach for clinical microbiology laboratories for routine susceptibility testing with simultaneous detection of ESBLs in Enterobacteriaceae.

* Corresponding author. Mailing address: Division of Microbiology, Calgary Laboratory Services and Department of Pathology & Laboratory Medicine, University of Calgary, 1638-10 Ave. SW, Calgary T3C 0J5, Canada. Phone: (403) 209-5309. Fax: (403) 209-5347. E-mail: johann.pitout{at}cls.ab.ca.

Journal of Clinical Microbiology, August 2003, p. 3933-3935, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3933-3935.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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