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Journal of Clinical Microbiology, August 2003, p. 3942-3944, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3942-3944.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Clinical Impact of a PCR Assay for Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Blood Cultures

M. Hallin,1* N. Maes,1 B. Byl,2 F. Jacobs,2 Y. De Gheldre,1,{dagger} and M. J. Struelens1

Laboratory of Microbiology,1 Infectious Diseases Clinic, Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium2

Received 31 January 2003/ Returned for modification 19 March 2003/ Accepted 22 May 2003

We evaluated the clinical usefulness of a PCR assay that discriminates Staphylococcus aureus from coagulase-negative staphylococci and detects methicillin resistance on blood cultures by measuring the adaptation of antimicrobial therapy based on the PCR results. Only 7 of 28 patients (25%) benefited from a modification of antibiotic therapy based on the PCR results, since empirical therapy was appropriate in a majority of cases.

* Corresponding author. Mailing address: Service de Microbiologie, Hôpital Erasme, 808 route de Lennik, 1070 Brussels, Belgium. Phone: 32 2 555 34 84. Fax: 32 2 555 31 10. E-mail: mahallin{at}ulb.ac.be.

{dagger} Present address: Laboratoire de Microbiologie, Cliniques Universitaires Mont-Godinne, 5530 Yvoir, Belgium.

Journal of Clinical Microbiology, August 2003, p. 3942-3944, Vol. 41, No. 8
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.8.3942-3944.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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