This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Prager, R.
Right arrow Articles by Tschäpe, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prager, R.
Right arrow Articles by Tschäpe, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2003, p. 4270-4278, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4270-4278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Properties of Salmonella enterica Serotype Paratyphi B Distinguish between Its Systemic and Its Enteric Pathovars

Rita Prager, Wolfgang Rabsch, Wiebke Streckel, Wolfgang Voigt, Erhardt Tietze, and Helmut Tschäpe*

Robert Koch Institute, Wernigerode, Germany

Received 4 February 2003/ Returned for modification 13 March 2003/ Accepted 25 June 2003

Salmonella enterica serotype O1,4,5,12:Hb:1,2, designated according to the current Kauffmann-White scheme as S. enterica serotype Paratyphi B, is a very diverse serotype with respect to its clinical and microbiological properties. PCR and blot techniques, which identify the presence, polymorphism, and expression of various effector protein genes, help to distinguish between strains with systemic and enteric outcomes of disease. All serotype Paratyphi B strains from systemic infections have been found to be somewhat genetically related with respect to the pattern of their virulence genes sopB, sopD, sopE1, avrA, and sptP as well as other molecular properties (multilocus enzyme electrophoresis type, pulsed-field gel electrophoresis [PFGE] type, ribotype, and IS200 type). They have been classified as members of the systemic pathovar (SPV). All these SPV strains possess a new sopE1-carrying bacteriophage (designated {Phi}SopE309) with high SopE1 protein expression but lack the commonly occurring avrA determinant. They exhibit normal SopB protein expression but lack SopD protein production. In contrast, strains from enteric infections classified as belonging to the enteric pathovar possess various combinations of the respective virulence genes, PFGE pattern, and ribotypes. We propose that the PCR technique for testing for the presence of the virulence genes sopE1 and avrA be used as a diagnostic tool for identifying both pathovars of S. enterica serotype Paratyphi B. This will be of great public health importance, since strains of serotype Paratyphi B have recently reemerged worldwide.

* Corresponding author. Mailing address: Robert Koch-Institut, Bereich Wernigerode, Burgstrasse 37, D-38855 Wernigerode, Germany. Phone: 49/39 43-67 92 37. Fax: 49/39 43-67 92 07. E-mail: tschaepeh{at}rki.de.

Journal of Clinical Microbiology, September 2003, p. 4270-4278, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4270-4278.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Djordjevic, S. P., Cain, A. K., Evershed, N. J., Falconer, L., Levings, R. S., Lightfoot, D., Hall, R. M. (2009). Emergence and Evolution of Multiply Antibiotic-Resistant Salmonella enterica Serovar Paratyphi B D-Tartrate-Utilizing Strains Containing SGI1. Antimicrob. Agents Chemother. 53: 2319-2326 [Abstract] [Full Text]  
  • Han, K. H., Choi, S. Y., Lee, J. H., Lee, H., Shin, E. H., Agtini, M. D., von Seidlein, L., Ochiai, R. L., Clemens, J. D., Wain, J., Hahn, J.-S., Lee, B. K., Song, M., Chun, J., Kim, D. W. (2006). Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356.. J Med Microbiol 55: 1661-1665 [Abstract] [Full Text]  
  • Harbottle, H., White, D. G., McDermott, P. F., Walker, R. D., Zhao, S. (2006). Comparison of Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates.. J. Clin. Microbiol. 44: 2449-2457 [Abstract] [Full Text]  
  • Tracz, D. M., Tabor, H., Jerome, M., Ng, L.-K., Gilmour, M. W. (2006). Genetic Determinants and Polymorphisms Specific for Human-Adapted Serovars of Salmonella enterica That Cause Enteric Fever.. J. Clin. Microbiol. 44: 2007-2018 [Abstract] [Full Text]  
  • Pelludat, C., Prager, R., Tschape, H., Rabsch, W., Schuchhardt, J., Hardt, W.-D. (2005). Pilot Study To Evaluate Microarray Hybridization as a Tool for Salmonella enterica Serovar Typhimurium Strain Differentiation. J. Clin. Microbiol. 43: 4092-4106 [Abstract] [Full Text]  
  • Weill, F.-X., Fabre, L., Grandry, B., Grimont, P. A. D., Casin, I. (2005). Multiple-Antibiotic Resistance in Salmonella enterica Serotype Paratyphi B Isolates Collected in France between 2000 and 2003 Is Due Mainly to Strains Harboring Salmonella Genomic Islands 1, 1-B, and 1-C. Antimicrob. Agents Chemother. 49: 2793-2801 [Abstract] [Full Text]  
  • Trosky, J. E., Mukherjee, S., Burdette, D. L., Roberts, M., McCarter, L., Siegel, R. M., Orth, K. (2004). Inhibition of MAPK Signaling Pathways by VopA from Vibrio parahaemolyticus. J. Biol. Chem. 279: 51953-51957 [Abstract] [Full Text]  
  • Porwollik, S., Boyd, E. F., Choy, C., Cheng, P., Florea, L., Proctor, E., McClelland, M. (2004). Characterization of Salmonella enterica Subspecies I Genovars by Use of Microarrays. J. Bacteriol. 186: 5883-5898 [Abstract] [Full Text]  
  • Hopkins, K. L., Threlfall, E. J. (2004). Frequency and polymorphism of sopE in isolates of Salmonella enterica belonging to the ten most prevalent serotypes in England and Wales. J Med Microbiol 53: 539-543 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»