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Journal of Clinical Microbiology, September 2003, p. 4353-4358, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4353-4358.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Corynebacterium nigricans sp. nov.: Proposed Name for a Black-Pigmented Corynebacterium Species Recovered from the Human Female Urogenital Tract

Sanjay K. Shukla,1* Kathryn A. Bernard,2 Mary Harney,3 Daniel N. Frank,4 and Kurt D. Reed1

Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin,1 Microbiology Laboratory, St. John's Hospital, Springfield, Illinois,3 Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado,4 National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada2

Received 13 March 2003/ Returned for modification 30 April 2003/ Accepted 27 June 2003

Six independent isolates of an unusual black-pigmented Corynebacterium species (strains CN-1, CN-2, CN-3415, W70124, 91-0032, and 92-0360) were recovered from the human female urogenital tract. Four of the six source patients had complications of pregnancy, including spontaneous abortion, preterm labor, and low amniotic fluid volume at the time of the pathogen isolation. One isolate was recovered from a vaginal ulcer. All six strains yielded black-pigmented colonies on sheep blood agar, chocolate agar, and colistin-nalidixic acid agar after 24 to 48 h of incubation at 35°C. The dry, adherent colonies pitted the agar surface. The cells were coccobacillary to rod-shaped, catalase positive, nonmotile, and nonlipophilic. Only five of six isolates were available for characterization. Biochemical and chemotaxonomic studies revealed that the strains belong to the genus Corynebacterium but differ from known corynebacterial species. Comparative 16S rRNA gene sequence analysis showed that the strains are closely related and form a new subline within the genus Corynebacterium. We propose the name Corynebacterium nigricans sp. nov. for this group of coryneforms. The type strain of Corynebacterium nigricans is CN-1. It is deposited in the American Type Culture Collection (assigned strain number ATCC 700975) and in the Institute Pasteur collection (assigned strain number CIP 107346).

* Corresponding author. Mailing address: Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave., Marshfield, WI 54449. Phone: (715) 389-5363. Fax: (715) 389-3808. E-mail: shukla.sanjay{at}mcrf.mfldclin.edu.

Journal of Clinical Microbiology, September 2003, p. 4353-4358, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4353-4358.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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