This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tortoli, E. Articles by Mazzarelli, G. Search for Related Content PubMed PubMed Citation Articles by Tortoli, E. Articles by Mazzarelli, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2003, p. 4418-4420, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4418-4420.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of INNO-LiPA MYCOBACTERIA v2: Improved Reverse Hybridization Multiple DNA Probe Assay for Mycobacterial Identification

Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory,¹ Cytogenetics and Genetics Unit,² Microbiological and Virological Serum-Immunology Laboratory, Careggi Hospital, Florence, Italy³

Received 3 March 2003/ Returned for modification 2 May 2003/ Accepted 6 June 2003

INNO-LiPA MYCOBACTERIA (Innogenetics, Ghent, Belgium) is a reverse hybridization DNA probe assay that has been recently improved by increasing the number of identifiable mycobacterial species to 16. Our assessment, performed with 197 mycobacteria belonging to 81 taxa, revealed 100% specificity and sensitivity for 20 out of 23 probes. The probes specific for Mycobacterium fortuitum complex, for the Mycobacterium avium-intracellulare-scrofulaceum group, and for Mycobacterium intracellulare type 2 cross-reacted with several mycobacteria rarely isolated from clinical specimens. The overall sensitivity was 100%, and the overall specificity was 94.4%.

This article has been cited by other articles:

• Turenne, C. Y., Wallace, R. Jr., Behr, M. A. (2007). Mycobacterium avium in the Postgenomic Era. Clin. Microbiol. Rev. 20: 205-229 [Abstract] [Full Text]  
• Xiong, L., Kong, F., Yang, Y., Cheng, J., Gilbert, G. L. (2006). Use of PCR and Reverse Line Blot Hybridization Macroarray Based on 16S-23S rRNA Gene Internal Transcribed Spacer Sequences for Rapid Identification of 34 Mycobacterium Species.. J. Clin. Microbiol. 44: 3544-3550 [Abstract] [Full Text]  
• Baptista, P. V., Koziol-Montewka, M., Paluch-Oles, J., Doria, G., Franco, R. (2006). Gold-Nanoparticle-Probe-Based Assay for Rapid and Direct Detection of Mycobacterium tuberculosis DNA in Clinical Samples.. Clin. Chem. 52: 1433-1434 [Full Text]  
• Russo, C., Tortoli, E., Menichella, D. (2006). Evaluation of the New GenoType Mycobacterium Assay for Identification of Mycobacterial Species. J. Clin. Microbiol. 44: 334-339 [Abstract] [Full Text]  
• Tortoli, E., Chianura, L., Fabbro, L., Mariottini, A., Martin-Casabona, N., Mazzarelli, G., Russo, C., Spinelli, M. (2005). Infections Due to the Newly Described Species Mycobacterium parascrofulaceum. J. Clin. Microbiol. 43: 4286-4287 [Abstract] [Full Text]  
• Lebrun, L., Weill, F.-X., Lafendi, L., Houriez, F., Casanova, F., Gutierrez, M. C., Ingrand, D., Lagrange, P., Vincent, V., Herrmann, J. L. (2005). Use of the INNO-LiPA-MYCOBACTERIA Assay (Version 2) for Identification of Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum Complex Isolates. J. Clin. Microbiol. 43: 2567-2574 [Abstract] [Full Text]  
• Zelazny, A. M., Calhoun, L. B., Li, L., Shea, Y. R., Fischer, S. H. (2005). Identification of Mycobacterium Species by secA1 Sequences. J. Clin. Microbiol. 43: 1051-1058 [Abstract] [Full Text]  
• Trueba, F., Fabre, M., Saint-Blancard, P. (2004). Rapid Identification of Mycobacterium genavense with a New Commercially Available Molecular Test, INNO-LiPA MYCOBACTERIA v2. J. Clin. Microbiol. 42: 4403-4404 [Abstract] [Full Text]  
• Piersimoni, C., Goteri, G., Nista, D., Mariottini, A., Mazzarelli, G., Bornigia, S. (2004). Mycobacterium lentiflavum as an Emerging Causative Agent of Cervical Lymphadenitis. J. Clin. Microbiol. 42: 3894-3897 [Abstract] [Full Text]  
• Tortoli, E., Rindi, L., Garcia, M. J., Chiaradonna, P., Dei, R., Garzelli, C., Kroppenstedt, R. M., Lari, N., Mattei, R., Mariottini, A., Mazzarelli, G., Murcia, M. I., Nanetti, A., Piccoli, P., Scarparo, C. (2004). Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov.. Int. J. Syst. Evol. Microbiol. 54: 1277-1285 [Abstract] [Full Text]  
• Padilla, E., Gonzalez, V., Manterola, J. M., Perez, A., Quesada, M. D., Gordillo, S., Vilaplana, C., Pallares, M. A., Molinos, S., Sanchez, M. D., Ausina, V. (2004). Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains. J. Clin. Microbiol. 42: 3083-3088 [Abstract] [Full Text]