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Journal of Clinical Microbiology, September 2003, p. 4438-4441, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4438-4441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a 5' Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Cariogenic Dental Pathogens Streptococcus mutans and Streptococcus sobrinus{dagger}

Akihiro Yoshida, Nao Suzuki, Yoshio Nakano,* Miki Kawada, Takahiko Oho, and Toshihiko Koga{ddagger}

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan

Received 26 February 2003/ Returned for modification 3 April 2003/ Accepted 29 May 2003

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81 92 642 6423. Fax: 81 92 642 6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.

{dagger} Respectfully dedicated to the memory of Toshihiko Koga, who passed away 14 October 2001.

{ddagger} Deceased.

Journal of Clinical Microbiology, September 2003, p. 4438-4441, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4438-4441.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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