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Journal of Clinical Microbiology, September 2003, p. 4460-4461, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4460-4461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Shell Vial Culture as a Tool for Isolation of Brucella melitensis in Chronic Hepatic Abscess

Clarisse Rovery,1 Jean Marc Rolain,2 Didier Raoult,2 and Philippe Brouqui1,2*

Service de Maladies Infectieuses et de Médecine Tropicale, Hôpital Nord, AHPM,1 Unité des Rickettsies, IFR 48, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, Marseille, France2

Received 24 April 2003/ Accepted 22 June 2003

A 50-year-old man was admitted with a liver abscess and positive serology for Brucella spp. Liver pus and blood cultures remained sterile on conventional culture. Inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded Brucella melitensis, identified by 16S rRNA gene amplification and sequencing.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385 Marseille, France. Phone: 33 491 32 43 75. Fax: 33 491 83 03 90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, September 2003, p. 4460-4461, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4460-4461.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Diaz, R., Ariza, J., Alberola, I., Casanova, A., Rubio, M. F. (2006). Secondary Serological Response of Patients with Chronic Hepatosplenic Suppurative Brucellosis. CVI 13: 1190-1196 [Abstract] [Full Text]  
  • Gouriet, F., Fenollar, F., Patrice, J.-Y., Drancourt, M., Raoult, D. (2005). Use of Shell-Vial Cell Culture Assay for Isolation of Bacteria from Clinical Specimens: 13 Years of Experience. J. Clin. Microbiol. 43: 4993-5002 [Abstract] [Full Text]  


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