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Journal of Clinical Microbiology, September 2003, p. 4465-4467, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4465-4467.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Multiplex PCR To Identify Staphylococcus aureus Adhesins Involved in Human Hematogenous Infections

Anne Tristan,1 Liu Ying,2 Michele Bes,1 Jerome Etienne,1 Francois Vandenesch,1 and Gerard Lina1*

Centre National de Référence des Staphylocoques, INSERM E0230, IFR62 Laennec, 69372 Lyon Cédex 08, France,1 Department of Clinical Laboratory, Xinhua Hospital, Shanghai 200092, China2

Received 23 March 2003/ Returned for modification 17 May 2003/ Accepted 3 June 2003

We have developed a multiplex PCR procedure to determine the distribution of nine adhesin genes in Staphylococcus aureus isolates. Only genes encoding bone sialoprotein binding protein and fibronectin binding protein B were significantly associated with hematogenous osteomyelitis/arthritis and native-valve endocarditis, respectively, suggesting their involvement in hematogenous tissue infections.

* Corresponding author. Mailing address: INSERM E0230, IFR62 Laennec, Rue Guillaume Paradin, 69372 Lyon Cédex 08, France. Phone: 33 478 77 86 57. Fax: 33 478 77 86 58. E-mail: geralina{at}univ-lyon1.fr.

Journal of Clinical Microbiology, September 2003, p. 4465-4467, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4465-4467.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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