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Journal of Clinical Microbiology, September 2003, p. 4471-4474, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4471-4474.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutations in katG, inhA, and ahpC Genes of Brazilian Isoniazid-Resistant Isolates of Mycobacterium tuberculosis

Márcia Susana N. Silva,1 Simone G. Senna,1,2 Marta O. Ribeiro,1,2 Andréia R. M. Valim,1,3 Maria Alice Telles,4 Afrânio Kritski,2 Glenn P. Morlock,5 Robert C. Cooksey,5 Arnaldo Zaha,3 and Maria Lucia R. Rossetti1,2*

Centro de Desenvolvimento Científico e Tecnológico, Laboratório Central de Saúde Pública, Fundação Estadual de Produção e Pesquisa em Saúde,1 Centro de Biotecnologia e Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre-Rio Grande do Sul,3 Setor de Micobactérias, Instituto Adolfo Lutz, São Paulo-SP,4 Instituto de Doenças do Tórax, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil,2 Division of AIDS, STD and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia5

Received 12 February 2003/ Returned for modification 17 March 2003/ Accepted 11 June 2003

The presence of mutations in specific regions of the katG, inhA, and ahpC genes was analyzed with 69 Mycobacterium tuberculosis isoniazid-resistant isolates from three Brazilian states. Point mutations in codon 315 of the katG gene were observed in 87.1, 60.9, and 60% of the isolates from Rio Grande do Sul, Rio de Janeiro, and São Paulo, respectively. Mutations in the inhA gene were identified only in one isolate from RJ State, and the ahpC promoter region revealed mutations in distinct positions in 12.9, 21.7, and 6.7% of the isolates from RS, RJ and SP, respectively.

* Corresponding author. Mailing address: Centro de Desenvolvimento Científico e Tecnológico/CDCT, Av. Ipiranga, 5400, CEP: 90610-000 Porto Alegre, RS, Brazil. Phone: 55 51 3352-0336. Fax: 55 51 3352-0336. E-mail: mrossett{at}terra.com.br.

Journal of Clinical Microbiology, September 2003, p. 4471-4474, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4471-4474.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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