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Journal of Clinical Microbiology, September 2003, p. 4475-4479, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4475-4479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Oral Peptostreptococcus Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes

Marcello P. Riggio* and Alan Lennon

Infection and Immunity Research Group, University of Glasgow Dental School, Glasgow G2 3JZ, United Kingdom

Received 2 December 2002/ Returned for modification 24 March 2003/ Accepted 2 June 2003

Oral Peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of PCR amplification of 16S rRNA genes and restriction enzyme analysis of the amplified products. This method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.

* Corresponding author. Mailing address: Level 9, Glasgow Dental Hospital & School, 378 Sauchiehall St., Glasgow G2 3JZ, Scotland, United Kingdom. Phone: 44 141 211 9742. Fax: 44 141 353 1593. E-mail: m.riggio{at}dental.gla.ac.uk.

Journal of Clinical Microbiology, September 2003, p. 4475-4479, Vol. 41, No. 9
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.9.4475-4479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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