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Journal of Clinical Microbiology, January 2004, p. 203-211, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.203-211.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diagnostic Assessment of Mycoplasma genitalium in Culture-Positive Women

Joel B. Baseman,1* Marianna Cagle,1 Jeffrey E. Korte,2 Caleb Herrera,1 Wanda G. Rasmussen,1 Janet G. Baseman,3 Rochelle Shain,2 and Jeanna M. Piper2

Department of Microbiology and Immunology,1 Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, Texas,2 Department of Epidemiology, University of Washington, Seattle, Washington3

Received 28 July 2003/ Returned for modification 7 September 2003/ Accepted 28 September 2003

Detection of Mycoplasma genitalium-mediated, chlamydia-negative nongonococcal urethritis and other M. genitalium-linked infectious etiologies has been very challenging. Although M. genitalium is considered a leading cause of genitourinary symptoms in men and women, extreme difficulties in its cultivation due to its highly fastidious nature and the lack of routine and effective diagnostic tests have slowed the generation of clinical data which directly implicate the presence of M. genitalium in disease pathogenesis. In this study, we compared enzyme-linked immunosorbent assays (ELISAs) and immunoblot and PCR assays in M. genitalium culture-positive women over 1 to 3 years of clinical visits to determine the usefulness of independent diagnostic strategies. Furthermore, the value of combinatorial diagnostic assessments is described, which provides insights into the dynamics of M. genitalium-host interactions. Overall, we show that neither ELISA nor PCR, alone or in combination, provides the sensitivity required to confidently predict the existence of viable M. genitalium organisms in cervical and vaginal samples. Additionally, culture-positive women exhibited a range of antibody responsiveness to M. genitalium based upon ELISA and immunoblot assessments, indicating immune diversity among this high-risk population.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, The University of Texas Health Science Center, 7703 Floyd Curl Dr., MC 7758, San Antonio, TX 78229-3900. Phone: (210) 567-3939. Fax: (210) 567-6491. E-mail: baseman{at}uthscsa.edu.

Journal of Clinical Microbiology, January 2004, p. 203-211, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.203-211.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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