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Journal of Clinical Microbiology, January 2004, p. 276-285, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.276-285.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparative Analysis of Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis for Characterizing Listeria monocytogenes Strains Isolated from Environmental and Clinical Sources

Tamara Revazishvili,{dagger} Mamuka Kotetishvili,{dagger} O. Colin Stine, Arnold S. Kreger, J. Glenn Morris Jr., and Alexander Sulakvelidze*

Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 27 June 2003/ Returned for modification 12 August 2003/ Accepted 18 September 2003

One hundred seventy-five Listeria monocytogenes strains were characterized by serotyping, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) based on loci in actA, betL, hlyA, gyrB, pgm, and recA. One hundred twenty-two sequence types (STs) were identified by MLST based on allelic profiles of the four housekeeping genes (betL, gyrB, pgm, and recA), and 34 and 38 alleles were identified for hlyA and actA, respectively. Several actA and hlyA alleles appeared to be predominantly associated with clinical isolates. MLST differentiated most of the L. monocytogenes strains better than did PFGE, and the discriminating ability of PFGE was better than that of serotyping. Several strains with different serotypes were found, by MLST and PFGE, to have very closely related genetic backgrounds, which suggested possible "antigen switching" among them. MLST can be a useful typing tool for differentiating L. monocytogenes strains (including strains undistinguishable by PFGE typing and serotyping), and it may be of value during investigations of food-borne outbreaks of listeriosis.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, MSTF Bldg., 10 South Pine St., Baltimore, MD 21201. Phone: (410) 706-4587. Fax: (410) 706-4581. E-mail: asulakve{at}epi.umaryland.edu.

{dagger} T.R. and M.K. contributed equally to the research described in this paper.

Journal of Clinical Microbiology, January 2004, p. 276-285, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.276-285.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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