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Detection of Severe Acute Respiratory Syndrome Coronavirus in Blood of Infected Patients

Genome Institute of Singapore,¹ DSO National Laboratories,² Environmental Health Institute, National Environment Agency,³ Tan Tock Seng Hospital,⁴ Department of Pathology, Singapore General Hospital and,⁵ Department of Microbiology, National University of Singapore, Singapore, Republic of Singapore⁶

Received 3 July 2003/ Returned for modification 9 September 2003/ Accepted 25 September 2003

Severe acute respiratory syndrome (SARS) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. PCR-based detection methods were developed for use on a variety of samples, including blood. Eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time PCR.

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