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Nissui Glucose Fermentative Gram-Negative Rod Identification System EB-20 Gives a Unique Profile for Typical Non-Sorbitol-Fermenting Escherichia coli O157:H7

Department of Microbiology, School of Medicine, Toho University, Otaku, Tokyo 143-8540,¹ Nissui Pharmaceutical Co. Ltd., Yuki, Ibaraki 307-0036, Japan²

Received 28 May 2003/ Returned for modification 10 September 2003/ Accepted 1 October 2003

The 98 non-sorbitol-fermenting (NSF) Escherichia coli O157:H7 strains identified on a Nissui glucose fermentative gram-negative rod identification system (EB-20) gave a unique biochemical profile number that was not detected in 85 pathogenic and 13 nonpathogenic E. coli strains. Thus, EB-20 is useful for the identification of NSF E. coli O157:H7 and provides a simple, cost-effective, and reliable tool for clinical laboratories.