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Journal of Clinical Microbiology, January 2004, p. 369-371, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.369-371.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Amplified Fragment Length Polymorphism Fingerprinting Is an Effective Technique To Distinguish Streptococcus pneumoniae from Other Streptococci and an Efficient Alternative to Pulsed-Field Gel Electrophoresis for Molecular Typing of Pneumococci

Chris Neeleman,1 Corné H. W. Klaassen,2* Hanneke A. de Valk,2 Maaike T. de Ruiter,2 and Johan W. Mouton2

Department of Intensive Care, Radboud University Hospital,1 Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands2

Received 18 July 2003/ Returned for modification 5 September 2003/ Accepted 6 October 2003

Amplified fragment length polymorphism versus pulsed-field gel electrophoresis was used for fingerprinting of 85 macrolide-resistant pneumococcal isolates identified by using primarily phenotypic methods. Confirmation of identification by 16S rRNA sequencing revealed that 27 isolates were actually nonpneumococci. Amplified fragment length polymorphism but not pulsed-field gel electrophoresis offered simultaneous and accurate discrimination between pneumococci and nonpneumococcal species.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg Door Jonkerbos 100, Nijmegen NL-6532 SZ, The Netherlands. Phone: 31-24-3657514. Fax: 31-24-3657516. E-mail: c.klaassen{at}cwz.nl.

Journal of Clinical Microbiology, January 2004, p. 369-371, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.369-371.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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