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Journal of Clinical Microbiology, January 2004, p. 372-377, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.372-377.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers

Roman Kotlowski,{dagger} Isdore Chola Shamputa, Nabil Abdullah El Aila, Anna Sajduda, Leen Rigouts, Armand van Deun, and Françoise Portaels*

Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium

Received 31 March 2003/ Returned for modification 2 June 2003/ Accepted 27 August 2003

In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5'-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5'-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS6110 allowed differentiation of M. tuberculosis isolates.

* Corresponding author. Mailing address: Prince Leopold Institute of Tropical Medicine, Mycobacteriology Unit, Department of Microbiology, Nationalestraat 155, 2000 Antwerp, Belgium. Phone: 32-3-247-63-17. Fax: 32-3-247-63-33. E-mail: portaels{at}itg.be.

{dagger} Present address: Faculty of Chemistry, Gdansk University of Technology, 80-952 Gdansk, Poland.

Journal of Clinical Microbiology, January 2004, p. 372-377, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.372-377.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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