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Journal of Clinical Microbiology, January 2004, p. 401-403, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.401-403.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Genome Selected Repeated Sequences Increases the Sensitivity of PCR Detection of Tropheryma whipplei

Florence Fenollar, Pierre-Edouard Fournier, Catherine Robert, and Didier Raoult*

Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de médecine, Université de la Méditerranée, 13385 Marseille cedex 05, France

Received 15 May 2003/ Returned for modification 10 July 2003/ Accepted 26 September 2003

The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02) without alteration of its specificity.

* Corresponding author. Mailing address: Unité des Rickettsies, IFR 48, Faculté de médecine, Université de la Méditerranée, 27, Blvd. Jean Moulin, 13385 Marseille cedex 05, France. Phone: (33) 04 91 38 55 17. Fax: (33) 04 91 83 03 90. E-mail: Didier.Raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, January 2004, p. 401-403, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.401-403.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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