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Journal of Clinical Microbiology, January 2004, p. 408-411, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.408-411.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

Petra Wolffs,1 Halfdan Grage,2 Oskar Hagberg,2 and Peter Rådström1*

Applied Microbiology, Lund Institute of Technology,1 Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden2

Received 30 July 2003/ Returned for modification 16 September 2003/ Accepted 14 October 2003

An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.

* Corresponding author. Mailing address: Applied Microbiology, Lund Institute of Technology, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Phone: 46 46-222 3412. Fax: 46 46-222 4203. E-mail: peter.radstrom{at}tmb.lth.se.

Journal of Clinical Microbiology, January 2004, p. 408-411, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.408-411.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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