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Journal of Clinical Microbiology, January 2004, p. 412-414, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.412-414.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Survival of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis Frozen in Skim Milk- Tryptone-Glucose-Glycerol Medium

Tarja Kaijalainen,1* Esa Ruokokoski,2 Pentti Ukkonen,2 and Elja Herva1

KTL, National Public Health Institute, Oulu,1 Helsinki, Finland2

Received 17 May 2002/ Returned for modification 29 September 2002/ Accepted 11 October 2003

In STGG (skim milk, tryptone, glucose, glycerol) medium at -80°C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in nasopharyngeal swabs for at least 1.5 years. At -20°C, S. pneumoniae and M. catarrhalis survived for 1.5 years, but H. influenzae survived for only 2 months.

* Corresponding author. Mailing address: KTL, National Public Health Institute, POB 310, FIN-90101 Oulu, Finland. Phone: 358-8 5376249. Fax: 358-8-5376251. E-mail: tarja.kaijalainen{at}ktl.fi.

Journal of Clinical Microbiology, January 2004, p. 412-414, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.412-414.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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