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Journal of Clinical Microbiology, January 2004, p. 421-425, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.421-425.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Strengths and Limitations of Commercial Tests for Hepatitis C Virus RNA Quantification

Chihiro Morishima,1,2 Minjun Chung,2 Ka Wing Ng,2 Donald J. Brambilla,4 and David R. Gretch2,3*

Departments of Pediatrics,1 Laboratory Medicine,2 Medicine, University of Washington School of Medicine, Seattle, Washington,3 New England Research Institutes, Watertown, Massachusetts4

Received 20 February 2003/ Returned for modification 17 April 2003/ Accepted 24 September 2003

The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.

* Corresponding author. Mailing address: 325 9th Ave., Box 359690, Harborview Medical Center, Seattle, WA 98104-2499. Phone: (206) 341-5216. Fax: (206) 341-5203. E-mail: gretch{at}u.washington.edu.

Journal of Clinical Microbiology, January 2004, p. 421-425, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.421-425.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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