Previous Article | Next Article 
Journal of Clinical Microbiology, January 2004, p. 421-425, Vol. 42, No. 1
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.1.421-425.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Strengths and Limitations of Commercial Tests for Hepatitis C Virus RNA Quantification
Chihiro Morishima,1,2 Minjun Chung,2 Ka Wing Ng,2 Donald J. Brambilla,4 and David R. Gretch2,3*
Departments of Pediatrics,1
Laboratory Medicine,2
Medicine, University of Washington School of Medicine, Seattle, Washington,3
New England Research Institutes, Watertown, Massachusetts4
Received 20 February 2003/
Returned for modification 17 April 2003/
Accepted 24 September 2003
The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.
* Corresponding author. Mailing address: 325 9th Ave., Box 359690, Harborview Medical Center, Seattle, WA 98104-2499. Phone: (206) 341-5216. Fax: (206) 341-5203. E-mail:
gretch{at}u.washington.edu.
Journal of Clinical Microbiology, January 2004, p. 421-425, Vol. 42, No. 1
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.1.421-425.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Chevaliez, S., Bouvier-Alias, M., Pawlotsky, J.-M.
(2009). Performance of the Abbott Real-Time PCR Assay Using m2000sp and m2000rt for Hepatitis C Virus RNA Quantification. J. Clin. Microbiol.
47: 1726-1732
[Abstract]
[Full Text]
-
Sabato, M. F., Shiffman, M. L., Langley, M. R., Wilkinson, D. S., Ferreira-Gonzalez, A.
(2007). Comparison of Performance Characteristics of Three Real-Time Reverse Transcription-PCR Test Systems for Detection and Quantification of Hepatitis C Virus. J. Clin. Microbiol.
45: 2529-2536
[Abstract]
[Full Text]
-
Scott, J. D., Gretch, D. R.
(2007). Molecular Diagnostics of Hepatitis C Virus Infection: A Systematic Review. JAMA
297: 724-732
[Abstract]
[Full Text]
-
Sarrazin, C., Gartner, B. C., Sizmann, D., Babiel, R., Mihm, U., Hofmann, W. P., von Wagner, M., Zeuzem, S.
(2006). Comparison of Conventional PCR with Real-Time PCR and Branched DNA-Based Assays for Hepatitis C Virus RNA Quantification and Clinical Significance for Genotypes 1 to 5.. J. Clin. Microbiol.
44: 729-737
[Abstract]
[Full Text]
-
Krajden, M., Shivji, R., Gunadasa, K., Mak, A., McNabb, G., Friesenhahn, M., Hendricks, D., Comanor, L.
(2004). Evaluation of the Core Antigen Assay as a Second-Line Supplemental Test for Diagnosis of Active Hepatitis C Virus Infection. J. Clin. Microbiol.
42: 4054-4059
[Abstract]
[Full Text]
-
Cook, L., Ng, K.-W., Bagabag, A., Corey, L., Jerome, K. R.
(2004). Use of the MagNA Pure LC Automated Nucleic Acid Extraction System followed by Real-Time Reverse Transcription-PCR for Ultrasensitive Quantitation of Hepatitis C Virus RNA. J. Clin. Microbiol.
42: 4130-4136
[Abstract]
[Full Text]
-
Pancoska, P., Moravek, Z., Moll, U. M.
(2004). Rational design of DNA sequences for nanotechnology, microarrays and molecular computers using Eulerian graphs. Nucleic Acids Res
32: 4630-4645
[Abstract]
[Full Text]
-
Barbeau, J. M., Goforth, J., Caliendo, A. M., Nolte, F. S.
(2004). Performance Characteristics of a Quantitative TaqMan Hepatitis C Virus RNA Analyte-Specific Reagent. J. Clin. Microbiol.
42: 3739-3746
[Abstract]
[Full Text]