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Journal of Clinical Microbiology, January 2004, p. 439-440, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.439-440.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Automated Procedure for Improving the RNA Isolation Step in Viral Load Testing for Human Immunodeficiency Virus

Min Xu, Yung Chan, Steven H. Fischer, and Alan T. Remaley*

Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508

Received 20 June 2003/ Accepted 25 September 2003

An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation procedure.

* Corresponding author. Mailing address: National Institutes of Health, Bldg. 10, Rm. 2C-433, 10 Center Dr., Bethesda, MD 20892-1508. Phone: (301) 402-9796. Fax: (301) 402-1885. E-mail: aremaley{at}nih.gov.

Journal of Clinical Microbiology, January 2004, p. 439-440, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.439-440.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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