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Journal of Clinical Microbiology, January 2004, p. 441-444, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.441-444.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of an Unusual Human Rotavirus Strain with G5P[8] Specificity in a Cameroonian Child with Diarrhea

Mathew D Esona,1 George E. Armah,1,2 Annelise Geyer,1 and A. Duncan Steele1*

MRC Diarrhoeal Pathogens Research Unit, MEDUNSA 0204, Pretoria, South Africa,1 Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana2

Received 14 August 2003/ Returned for modification 3 October 2003/ Accepted 23 October 2003

Rotavirus strains detected as part of ongoing strain surveillance in Cameroon, and whose first-round reverse transcription-PCR product could not be genotyped by using conventional genotyping primers, were subjected to sequence analysis for strain characterization. We detected for the first time in Africa a human rotavirus with G5 specificity. The Cameroonian G5 strain had a short electrophoretic pattern and was of VP6 subgroup I specificity and a VP4 P[8] type. The VP7 gene shared a higher nucleic acid and amino acid homology with the porcine G5 strain CC117 (90 and 96%, respectively) than with human G5 strain IAL-28 (86 and 92%, respectively). Phylogenetic analysis showed Cameroonian strain MRC3105 clustered together in the same lineage as two other reported porcine G5 strains. The Cameroonian G5 strain, the first to be reported in humans outside of Latin America, may be a natural reassortant between animal and human rotavirus strains.

* Corresponding author. Mailing address: MRC/MEDUNSA Diarrhoeal Pathogens Research Unit, Medical University of Southern Africa, Pretoria, Republic of South Africa. Phone: 27 12 521-5938. Fax: 27 12 521 5794. E-mail: adsteele@medunsa.co.za; steeled{at}who.int.

Journal of Clinical Microbiology, January 2004, p. 441-444, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.441-444.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sharma, S., Ray, P., Gentsch, J. R., Glass, R. I., Kalra, V., Bhan, M. K. (2008). Emergence of G12 Rotavirus Strains in Delhi, India, in 2000 to 2007. J. Clin. Microbiol. 46: 1343-1348 [Abstract] [Full Text]  


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