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Journal of Clinical Microbiology, January 2004, p. 449-452, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.449-452.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Serodiagnosis of Chronic and Acute Chagas' Disease with Trypanosoma cruzi Recombinant Proteins: Results of a Collaborative Study in Six Latin American Countries

Eufrosina S. Umezawa,1* Alejandro O. Luquetti,2 Gabriela Levitus,3 Carlos Ponce,4 Elisa Ponce,4 Diana Henriquez,5 Susana Revollo,6 Bertha Espinoza,7 Octavio Sousa,8 Baldip Khan,9 and José Franco da Silveira10

Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo,1 Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, UNIFESP, São Paulo,10 Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiania, Brazil,2 Instituto de Investigaciones en Engenieria Genética y Biologia Molecular (INGEBI), Buenos Aires, Argentina,3 Laboratorio de Referencia para Enfermedad de Chagas y Leishmaniasis, Secretaria de la Salud, Tegucigalpa, Honduras,4 Departamento de Ciencias Biologicas, Universidad Simon Bolivar, Caracas, Venezuela,5 Instituto de Servicios de Laboratório de Diagnóstico e Investigacion en Salud (Seladis), La Paz, Bolívia,6 Departamento de Inmunologia, Instituto de Investigaciones Biomédicas, Universidad Autonoma de Mexico, Mexico DF, México,7 Centro de Investigacion y Diagnóstico de Enfermedades Parasitárias, Facultad de Medicina, Universidad de Panama, Panama, Panama,8 Division of Human Health, International Atomic Energy Agency, Vienna, Austria9

Received 17 June 2003/ Returned for modification 21 July 2003/ Accepted 23 September 2003

An enzyme-linked immunosorbent assay to diagnose Chagas' disease by a serological test was performed with Trypanosoma cruzi recombinant antigens (JL8, MAP, and TcPo). High sensitivity (99.4%) and specificity (99.3%) were obtained when JL8 was combined with MAP (JM) and tested with 150 serum samples from chagasic and 142 nonchagasic individuals. Moreover, JM also diagnosed 84.2% of patients in the acute phase of T. cruzi infection.

* Corresponding author. Mailing address: Instituto de Medicina Tropical de São Paulo da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar 470, CEP 05403-000, São Paulo, SP, Brazil. Phone: 55-113066 7015. Fax: 55-113088 5237. E-mail: eumezawa{at}usp.br.

Journal of Clinical Microbiology, January 2004, p. 449-452, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.449-452.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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