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Journal of Clinical Microbiology, January 2004, p. 7-11, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.7-11.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Susceptibility Testing of Enterobacteriaceae and Pseudomonas aeruginosa by Direct Inoculation from Positive BACTEC Blood Culture Bottles into Vitek 2

Marjan J. Bruins,* Peter Bloembergen, Gijs J. H. M. Ruijs, and Maurice J. H. M. Wolfhagen

Laboratory of Clinical Microbiology and Infectious Diseases, Isala Klinieken, 8021 AM Zwolle, The Netherlands

Received 29 August 2003/ Returned for modification 26 September 2003/ Accepted 7 October 2003

Inoculation of an automated system for rapid identification (ID) and antimicrobial susceptibility testing (AST) directly from positive blood culture bottles will reduce the turnaround time of laboratory diagnosis of septicemic patients, which benefits clinical outcome and decreases patient costs. Direct test results, however, must always be confirmed by testing a pure overnight culture, which is the "gold standard." We studied the accuracy of direct testing versus repeat testing in order to investigate the possibility of refraining from repeat testing. We also assessed the clinical risk of reporting results based on direct testing only. We inoculated Vitek 2 (bioMérieux) directly from 410 positive BACTEC 9240 (BD) blood culture bottles containing gram-negative rods and studied the ID and AST results. In a comparison of direct inoculation with the standard method, a total of 344 isolates of Enterobacteriaceae and Pseudomonas aeruginosa were tested, and 93.0% were correctly identified. Of the 39 (10.2%) samples that contained bacilli not identifiable by Vitek 2, only 1 gave a conclusive, correct result. The overall MIC agreement among 312 isolates was 99.2%, with 0.8% very major and 0.02% major error rates. Of only three (polymicrobial) samples, the direct susceptibility pattern would be reported to the clinician as too sensitive. Vitek 2 results obtained from direct inoculation of blood culture bottles containing gram-negative bacilli are safe enough for immediate reporting, provided that ID and AST are consistent. Repeat testing is not necessary, unless Gram stain or overnight subculture results raise doubt about the purity of the culture.

* Corresponding author. Mailing address: Isala Klinieken, Laboratory of Clinical Microbiology and Infectious Diseases, Rhijnvis Feithlaan 62, 8021 AM Zwolle, The Netherlands. Phone: 31-38-424-3111. Fax: 31-38-424-3146. E-mail: m.j.bruins{at}isala.nl.

Journal of Clinical Microbiology, January 2004, p. 7-11, Vol. 42, No. 1
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.1.7-11.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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