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Journal of Clinical Microbiology, October 2004, p. 4444-4448, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4444-4448.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Origins of Microsatellite Diversity in the Trichophyton rubrum- T. violaceum Clade (Dermatophytes)

T. Ohst,1 S. de Hoog,2 W. Presber,1 V. Stavrakieva,3 and Y. Gräser1*

Department of Parasitology (Charité), Institut für Mikrobiologie und Hygiene, Humboldt University, Berlin, Germany,1 Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands,2 Department of Microbiology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria3

Received / Returned for modification 7 May 2004/ Accepted 29 June 2004

We analyzed the population structure of the anthropophilic dermatophyte species Trichophyton violaceum, which mainly causes tinea capitis, and T. rubrum, the most frequently isolated agent of dermatophytosis worldwide. A microsatellite marker (T1) was developed by using the enrichment technique for microsatellites. The T1 marker containing a (GT)8-10 repeat was proven to specifically amplify both species, underlining their close kinship. Four polymorphic alleles were detected within a set of about 130 strains by using polyacrylamide gel electrophoresis with this marker. An association with geographic origin of the isolates was apparent. Given the close relatedness of both species, these data suggest an African origin of the entire T. rubrum complex, followed by the emergence of a new genotype (B) in Asia with subsequent spread of this genotype over Europe and the United States.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Campus Mitte, Humboldt Universität zu Berlin, Dorotheenstr. 96, D-10117 Berlin, Germany. Fax: 49-30-450-524902. Phone: 49-30-450-524066. E-mail: yvonne.graeser{at}charite.de.

Journal of Clinical Microbiology, October 2004, p. 4444-4448, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4444-4448.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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