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Journal of Clinical Microbiology, October 2004, p. 4494-4497, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4494-4497.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotyping of 44 Isolates of Fusarium solani, the Main Agent of Fungal Keratitis in Brazil

Patricio Godoy,1 Josep Cano,2 Josepa Gené,2 Josep Guarro,2* Ana Luisa Höfling-Lima,3 and Arnaldo Lopes Colombo1

Disciplina de Doenças Infecciosas e Parasitárias (DIPA),1 Departamento de Oftalmologia, Universidade Federal de São Paulo, Brazil,3 Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain2

Received 5 April 2004/ Returned for modification 3 May 2004/ Accepted 13 June 2004

In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.

* Corresponding author. Mailing address: Unitat de Microbiologia, Departament de Ciències Mèdiques Bàsiques, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201-Reus, Tarragona, Spain. Phone: 34 977759359. Fax: 34 977759322. E-mail: jgd{at}fmcs.urv.es.

Journal of Clinical Microbiology, October 2004, p. 4494-4497, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4494-4497.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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