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Genotyping of 44 Isolates of Fusarium solani, the Main Agent of Fungal Keratitis in Brazil

Disciplina de Doenças Infecciosas e Parasitárias (DIPA),¹ Departamento de Oftalmologia, Universidade Federal de São Paulo, Brazil,³ Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain²

Received 5 April 2004/ Returned for modification 3 May 2004/ Accepted 13 June 2004

In a retrospective study performed over 6 years in Brazil, Fusarium solani was found to be the most common species causing mycotic keratitis. The genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and PCR restriction fragment length polymorphism (PCR-RFLP) fingerprinting. ERIC-PCR was more discriminatory than PCR-RFLP for differentiating the strains. By combining of the results of both techniques, we identified 40 genotypes. Molecular typing revealed a high genomic heterogeneity of the strains of F. solani studied.

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