This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perry, J. D.
Right arrow Articles by Gould, F. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perry, J. D.
Right arrow Articles by Gould, F. K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2004, p. 4519-4523, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4519-4523.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a Chromogenic Agar Medium for Methicillin-Resistant Staphylococcus aureus

John D. Perry,* Amie Davies, Lynne A. Butterworth, Andrew L. J. Hopley, Audrey Nicholson, and F. Kate Gould

Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom

Received 9 April 2004/ Accepted 21 June 2004

We describe here the development and evaluation of MRSA ID, a new chromogenic agar medium for the specific isolation and identification of methicillin-resistant Staphylococcus aureus (MRSA). We used S. aureus ID (bioMérieux, La Balme Les Grottes, France) and supplemented it with various antimicrobials, including cefoxitin, ciprofloxacin, oxacillin, and methicillin. Cefoxitin proved to be superior to the other antimicrobials for the selection of MRSA from other strains of S. aureus. MRSA ID (consisting of S. aureus ID supplemented with 4 mg of cefoxitin/liter) was evaluated by the use of 747 swabs from various clinical sites. All specimens were also cultured on CHROMagar MRSA and oxacillin resistance screening agar base (ORSAB) and in selective mannitol broth (SMB). A total of 85 MRSA strains were isolated by a combination of all methods. After 22 to 24 h of incubation, 80% of the MRSA strains were isolated as green colonies on MRSA ID, compared with 59 and 62% of the strains that were isolated as colored colonies on CHROMagar MRSA and ORSAB, respectively. After 48 h of incubation, 89, 72, and 78% of the MRSA strains were isolated on MRSA ID, CHROMagar MRSA, and ORSAB, respectively. Sixty-five percent of the strains were isolated by growth in SMB. The specificities of MRSA ID, CHROMagar MRSA, ORSAB, and SMB were 99.5, 99.3, 97.9, and 92.8%, respectively, after 22 to 24 h of incubation. We conclude that MRSA ID is a sensitive and specific medium for the isolation and identification of MRSA.

* Corresponding author. Mailing address: Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, Tyne and Wear NE7 7DN, United Kingdom. Phone: 44 (191) 2843111, ext. 26691. Fax: 44 (191) 2231224. E-mail: jdp{at}blueyonder.co.uk.

Journal of Clinical Microbiology, October 2004, p. 4519-4523, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4519-4523.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Paule, S. M., Mehta, M., Hacek, D. M., Gonzalzles, T.-M., Robicsek, A., Peterson, L. R. (2009). Chromogenic Media vs Real-Time PCR for Nasal Surveillance of Methicillin-Resistant Staphylococcus aureus: Impact on Detection of MRSA-Positive Persons. Am J Clin Pathol 131: 532-539 [Abstract] [Full Text]  
  • Carson, J., Lui, B., Rosmus, L., Rennick, H., Fuller, J. (2009). Interpretation of MRSASelect Screening Agar at 24 Hours of Incubation. J. Clin. Microbiol. 47: 566-568 [Abstract] [Full Text]  
  • Krishna, B V S, Smith, M, McIndeor, A, Gibb, A P, Dave, J (2008). Evaluation of Chromogenic MRSA medium, MRSASelect and Oxacillin Resistance Screening Agar for the detection of methicillin-resistant Staphylococcus aureus. J. Clin. Pathol. 61: 841-843 [Abstract] [Full Text]  
  • Malhotra-Kumar, S., Haccuria, K., Michiels, M., Ieven, M., Poyart, C., Hryniewicz, W., Goossens, H., on behalf of the MOSAR WP2 Study Team, (2008). Current Trends in Rapid Diagnostics for Methicillin-Resistant Staphylococcus aureus and Glycopeptide-Resistant Enterococcus Species. J. Clin. Microbiol. 46: 1577-1587 [Full Text]  
  • Boyce, J. M., Havill, N. L. (2008). Comparison of BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA Assay for Screening Patients for the Presence of MRSA Strains. J. Clin. Microbiol. 46: 350-351 [Abstract] [Full Text]  
  • van Loo, I. H. M., van Dijk, S., Verbakel-Schelle, I., Buiting, A. G. M. (2007). Evaluation of a chromogenic agar (MRSASelect) for the detection of meticillin-resistant Staphylococcus aureus with clinical samples in The Netherlands. J Med Microbiol 56: 491-494 [Abstract] [Full Text]  
  • Cherkaoui, A., Renzi, G., Francois, P., Schrenzel, J. (2007). Comparison of four chromogenic media for culture-based screening of meticillin-resistant Staphylococcus aureus. J Med Microbiol 56: 500-503 [Abstract] [Full Text]  
  • Glupczynski, Y., Berhin, C., Bauraing, C., Bogaerts, P. (2007). Evaluation of a New Selective Chromogenic Agar Medium for Detection of Extended-Spectrum {beta}-Lactamase-Producing Enterobacteriaceae. J. Clin. Microbiol. 45: 501-505 [Abstract] [Full Text]  
  • Compernolle, V., Verschraegen, G., Claeys, G. (2007). Combined Use of Pastorex Staph-Plus and Either of Two New Chromogenic Agars, MRSA ID and CHROMagar MRSA, for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 154-158 [Abstract] [Full Text]  
  • Han, Z., Lautenbach, E., Fishman, N., Nachamkin, I. (2007). Evaluation of mannitol salt agar, CHROMagar Staph aureus and CHROMagar MRSA for detection of meticillin-resistant Staphylococcus aureus from nasal swab specimens. J Med Microbiol 56: 43-46 [Abstract] [Full Text]  
  • Louie, L., Soares, D., Meaney, H., Vearncombe, M., Simor, A. E. (2006). Evaluation of a New Chromogenic Medium, MRSA Select, for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44: 4561-4563 [Abstract] [Full Text]  
  • Rosenthal, A., White, D., Churilla, S., Brodie, S., Katz, K. C. (2006). Optimal Surveillance Culture Sites for Detection of Methicillin-Resistant Staphylococcus aureus in Newborns. J. Clin. Microbiol. 44: 4234-4236 [Abstract] [Full Text]  
  • Fang, H., Hedin, G. (2006). Use of Cefoxitin-Based Selective Broth for Improved Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44: 592-594 [Abstract] [Full Text]  
  • Stoakes, L., Reyes, R., Daniel, J., Lennox, G., John, M. A., Lannigan, R., Hussain, Z. (2006). Prospective Comparison of a New Chromogenic Medium, MRSASelect, to CHROMagar MRSA and Mannitol-Salt Medium Supplemented with Oxacillin or Cefoxitin for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 44: 637-639 [Abstract] [Full Text]  
  • Brown, D. F. J., Edwards, D. I., Hawkey, P. M., Morrison, D., Ridgway, G. L., Towner, K. J., Wren, M. W. D., on behalf of the Joint Working Party of the Britis, (2005). Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56: 1000-1018 [Abstract] [Full Text]  
  • Smyth, R. W., Kahlmeter, G. (2005). Mannitol Salt Agar-Cefoxitin Combination as a Screening Medium for Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 3797-3799 [Abstract] [Full Text]  
  • Hedin, G., Fang, H. (2005). Evaluation of Two New Chromogenic Media, CHROMagar MRSA and S. aureus ID, for Identifying Staphylococcus aureus and Screening Methicillin-Resistant S. aureus. J. Clin. Microbiol. 43: 4242-4244 [Abstract] [Full Text]  
  • Diederen, B., van Duijn, I., van Belkum, A., Willemse, P., van Keulen, P., Kluytmans, J. (2005). Performance of CHROMagar MRSA Medium for Detection of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 1925-1927 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»