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Journal of Clinical Microbiology, October 2004, p. 4556-4565, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4556-4565.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiological Analysis of Mycoplasma bovis Isolates from the United Kingdom Shows Two Genetically Distinct Clusters

Laura McAuliffe,1* Branko Kokotovic,2 Roger D. Ayling,1 and Robin A. J. Nicholas1

Mycoplasma Group, Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency (Weybridge), Surrey, United Kingdom,1 Danish Veterinary Laboratory, Copenhagen, Denmark2

Received 15 April 2004/ Returned for modification 14 June 2004/ Accepted 7 July 2004

Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected in the United Kingdom between 1996 and 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and random amplified polymorphic DNA (RAPD) analysis. In addition, the influence of variable surface protein (Vsp) profiles on the profiles generated with molecular typing techniques was studied. Both AFLP and RAPD separated the isolates into two distinct groups, but PFGE showed less congruence with the other techniques. There was no clear relationship between the geographic origin or year of isolation of the isolates and the profiles produced. No correlation between Vsp profiles and any of the molecular typing techniques was observed. We propose that RAPD and AFLP provide valuable tools for molecular typing of M. bovis.

* Corresponding author. Mailing address: Mycoplasma Group, Department of Statutory and Exotic Bacteria, Veterinary Laboratories Agency (Weybridge), Woodham Lane, Addlestone, Surrey, United Kingdom KT15 3NB. Phone: (44) 1932 357918. Fax: (44) 1932 357423. E-mail: l.mcauliffe{at}vla.defra.gsi.gov.uk.

Journal of Clinical Microbiology, October 2004, p. 4556-4565, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4556-4565.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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