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Journal of Clinical Microbiology, October 2004, p. 4577-4580, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4577-4580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Plate for Antifungal Susceptibility Testing of the New Triazoles Voriconazole, Posaconazole, and Ravuconazole

M. A. Pfaller,1* A. Espinel-Ingroff,2 and R. N. Jones3,4

University of Iowa College of Medicine and College of Public Health, Iowa City, Iowa,1 Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia,2 JMI Laboratories, North Liberty, Iowa,3 Tufts University School of Medicine, Boston, Massachusetts4

Received 19 April 2004/ Returned for modification 27 May 2004/ Accepted 13 June 2004

A commercially prepared dried colorimetric microdilution panel (Sensititre YeastOne, TREK Diagnostic Systems, Cleveland, Ohio) was compared in three different laboratories with the National Committee for Clinical Laboratory Standards (NCCLS) reference microdilution method by testing two quality control strains and 300 clinical isolates of Candida spp. against fluconazole, voriconazole, posaconazole, and ravuconazole. Reference MIC endpoints were established after 48 h of incubation and YeastOne colorimetric endpoints were established after 24 h of incubation. YeastOne endpoints were determined to be the lowest concentration at which the color in the well changed from red (indicating growth) to purple (indicating growth inhibition) or blue (indicating no growth). Excellent agreement (within two dilutions) between the reference and colorimetric MICs was observed. Overall agreement was 95.4%. Agreement ranged from 92.3% with posaconazole to 98.0% with fluconazole. The YeastOne colorimetric method appears to be comparable to the NCCLS reference method for testing the susceptibility of Candida spp to the new triazoles voriconazole, posaconazole, and ravuconazole.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 384-9566. Fax: (319) 356-4916. E-mail: michael-pfaller{at}uiowa.edu.

Journal of Clinical Microbiology, October 2004, p. 4577-4580, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4577-4580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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